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Literature summary extracted from

  • Li, Z.; Gao, R.; Hao, Q.; Zhao, H.; Cheng, L.; He, F.; Liu, L.; Liu, X.; Chou, W.K.; Zhu, H.; Cane, D.E.
    The T296V mutant of amorpha-4,11-diene synthase is defective in allylic diphosphate isomerization but retains the ability to cyclize the intermediate (3R)-nerolidyl diphosphate to amorpha-4,11-diene (2016), Biochemistry, 55, 6599-6604 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.24 expressed in Escherichia coli Rosetta(DE3) cells Artemisia annua

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.24 T296A the mutant predominantly retains amorphadiene synthase activity by converting (2E,6E)-farnesyl diphosphate to 78% amorpha-4,11-diene and 5% (E)-beta-farnesene Artemisia annua
4.2.3.24 T296I the mutant converts (2E,6E)-farnesyl diphosphate to 100% (E)-beta-farnesene Artemisia annua
4.2.3.24 T296L the mutant converts (2E,6E)-farnesyl diphosphate to 35.24% amorpha-4,11-diene and 54.05% (E)-beta-farnesene Artemisia annua
4.2.3.24 T296S the mutant converts (2E,6E)-farnesyl diphosphate to 90.32% amorpha-4,11-diene and 1.57% (E)-beta-farnesene Artemisia annua
4.2.3.24 T296V the mutant is defective in allylic diphosphate isomerization but retains the ability to cyclize the intermediate (3R,6E)-nerolidyl diphosphate to amorpha-4,11-diene. The mutant catalyzes the abortive conversion of (2E,6E)-farnesyl diphosphate mainly into the acyclic product (E)-beta-farnesene (88%) Artemisia annua

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.24 0.0108
-
 (2E,6E)-farnesyl diphosphate mutant enzyme T296A, at pH 7.2 and 30°C Artemisia annua
4.2.3.24 0.0153
-
 (2E,6E)-farnesyl diphosphate wild type enzyme, at pH 7.2 and 30°C Artemisia annua
4.2.3.24 0.0198
-
 (2E,6E)-farnesyl diphosphate mutant enzyme T296I, at pH 7.2 and 30°C Artemisia annua
4.2.3.24 0.0282
-
 (2E,6E)-farnesyl diphosphate mutant enzyme T296V, at pH 7.2 and 30°C Artemisia annua
4.2.3.24 0.033
-
 (2E,6E)-farnesyl diphosphate mutant enzyme T296S, at pH 7.2 and 30°C Artemisia annua

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.24 Mg2+ required Artemisia annua

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.24 (2E,6E)-farnesyl diphosphate Artemisia annua
-
 amorpha-4,11-diene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.24 Artemisia annua Q9AR04
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.24 Ni-NTA column chromatography Artemisia annua

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.24 (2E,6E)-farnesyl diphosphate
-
 Artemisia annua amorpha-4,11-diene + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.24 ADS
-
 Artemisia annua
4.2.3.24 amorphadiene synthase
-
 Artemisia annua

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.24 0.0013
-
 (2E,6E)-farnesyl diphosphate mutant enzyme T296I, at pH 7.2 and 30°C Artemisia annua
4.2.3.24 0.0015
-
 (2E,6E)-farnesyl diphosphate mutant enzyme T296V, at pH 7.2 and 30°C Artemisia annua
4.2.3.24 0.0059
-
 (2E,6E)-farnesyl diphosphate mutant enzyme T296A, at pH 7.2 and 30°C Artemisia annua
4.2.3.24 0.0075
-
 (2E,6E)-farnesyl diphosphate mutant enzyme T296S, at pH 7.2 and 30°C Artemisia annua
4.2.3.24 0.0163
-
 (2E,6E)-farnesyl diphosphate wild type enzyme, at pH 7.2 and 30°C Artemisia annua

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.24 0.05
-
 (2E,6E)-farnesyl diphosphate mutant enzyme T296V, at pH 7.2 and 30°C Artemisia annua
4.2.3.24 0.07
-
 (2E,6E)-farnesyl diphosphate mutant enzyme T296I, at pH 7.2 and 30°C Artemisia annua
4.2.3.24 0.23
-
 (2E,6E)-farnesyl diphosphate mutant enzyme T296S, at pH 7.2 and 30°C Artemisia annua
4.2.3.24 0.55
-
 (2E,6E)-farnesyl diphosphate mutant enzyme T296A, at pH 7.2 and 30°C Artemisia annua
4.2.3.24 1.06
-
 (2E,6E)-farnesyl diphosphate wild type enzyme, at pH 7.2 and 30°C Artemisia annua