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Literature summary extracted from

  • Neti, S.S.; Eckert, D.M.; Poulter, C.D.
    Construction of functional monomeric type 2 isopentenyl diphosphate dimethylallyl diphosphate isomerase (2016), Biochemistry, 55, 4229-4238 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.2 expressed in Escherichia coli M15pREP4 cells Streptococcus pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
5.3.3.2 N37A the mutant is a monomeric but active enzyme with by 20°C lowered melting temperature and reduced binding affinities of FMN (40fold) and a minimal effect on the kcat value compared to the wild type enzyme Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.3.2 FMN
-
Streptococcus pneumoniae
5.3.3.2 isopentenyl diphosphate
-
Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.3.2 0.00595
-
isopentenyl diphosphate wild type enzyme, at anaerobic conditions, at pH 7.0 and 37°C Streptococcus pneumoniae
5.3.3.2 0.0408
-
isopentenyl diphosphate wild type enzyme, at aerobic conditions, at pH 7.0 and 37°C Streptococcus pneumoniae
5.3.3.2 0.572
-
isopentenyl diphosphate mutant enzyme N37A, at aerobic conditions, at pH 7.0 and 37°C Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.3.2 isopentenyl diphosphate Streptococcus pneumoniae
-
dimethylallyl diphosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.2 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.2 Ni-NTA agarose resin column chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.2 isopentenyl diphosphate
-
Streptococcus pneumoniae dimethylallyl diphosphate
-
r

Subunits

EC Number Subunits Comment Organism
5.3.3.2 homotetramer
-
Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
5.3.3.2 Idi-2
-
Streptococcus pneumoniae
5.3.3.2 type 2 isopentenyl diphosphate:dimethylallyl diphosphate isomerase
-
Streptococcus pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.3.2 0.875
-
isopentenyl diphosphate wild type enzyme, at anaerobic conditions, at pH 7.0 and 37°C Streptococcus pneumoniae
5.3.3.2 0.983
-
isopentenyl diphosphate wild type enzyme, at aerobic conditions, at pH 7.0 and 37°C Streptococcus pneumoniae
5.3.3.2 1.31
-
isopentenyl diphosphate mutant enzyme N37A, at aerobic conditions, at pH 7.0 and 37°C Streptococcus pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.3.2 FMN
-
Streptococcus pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.3.2 0.0064
-
FMN wild type enzyme, at anaerobic conditions, at pH 7.0 and 37°C Streptococcus pneumoniae
5.3.3.2 0.292
-
isopentenyl diphosphate wild type enzyme, at aerobic conditions, at pH 7.0 and 37°C Streptococcus pneumoniae