Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Taberman, H.; Andberg, M.; Parkkinen, T.; Jänis, J.; Penttilä, M.; Hakulinen, N.; Koivula, A.; Rouvinen, J.
    Structure and function of a decarboxylating Agrobacterium tumefaciens keto-deoxy-D-galactarate dehydratase (2014), Biochemistry, 53, 8052-8060 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.41 expression in Escherichia coli Agrobacterium fabrum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.41 crystal structures of keto-deoxy-d-galactarate dehydratase and its complexes with pyruvate and 2-oxoadipic acid, two substrate analogues, are determined to 1.7 A, 1.5 A, and 2.1 A resolution, respectively Agrobacterium fabrum
4.2.1.41 crystals are grown by the hanging drop vapor diffusion method at 20°C, crystal structures are determined to 1.7 A, 1.5 A, and 2.1 A resolution, respectively Agrobacterium fabrum

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.41 S211A the specific activity of the purified Ser211Ala mutant enzyme is approximately 30 times lower than the activity of the wild-type enzyme Agrobacterium fabrum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.41 0.5
-
 5-dehydro-4-deoxy-D-glucarate pH 7.5, 22°C Agrobacterium fabrum

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.41 Agrobacterium fabrum Q8UB77
-
-
4.2.1.41 Agrobacterium fabrum ATCC 33970 Q8UB77
-
-
4.2.1.41 Agrobacterium fabrum C58 Q8UB77
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.41
-
 Agrobacterium fabrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.41 5-dehydro-4-deoxy-D-glucarate i.e. 3-deoxy-L-threo-2-hexulosarate Agrobacterium fabrum 2,5-dioxopentanoate + CO2
-
 ?
4.2.1.41 5-dehydro-4-deoxy-D-glucarate i.e. 3-deoxy-L-threo-2-hexulosarate Agrobacterium fabrum C58 2,5-dioxopentanoate + CO2
-
 ?
4.2.1.41 5-dehydro-4-deoxy-D-glucarate i.e. 3-deoxy-L-threo-2-hexulosarate Agrobacterium fabrum ATCC 33970 2,5-dioxopentanoate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.41 KDG dehydratase
-
 Agrobacterium fabrum
4.2.1.41 keto-deoxy-D-galactarate dehydratase
-
 Agrobacterium fabrum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.41 40
-
 the enzyme retains more than 60% of its activity after 22 h Agrobacterium fabrum
4.2.1.41 50
-
 activity is reduced to 20% after 22 h Agrobacterium fabrum
4.2.1.41 70
-
 less than 1% activity is detected after 30 min Agrobacterium fabrum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.41 8.83
-
 5-dehydro-4-deoxy-D-glucarate pH 7.5, 22°C Agrobacterium fabrum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.41 7.5 8
-
 Agrobacterium fabrum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.41 17
-
 5-dehydro-4-deoxy-D-glucarate pH 7.5, 22°C Agrobacterium fabrum