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Literature summary extracted from

  • Belogurov, G.; Malinen, A.; Turkina, M.; Jalonen, U.; Rytkönen, K.; Baykov, A.; Lahti, R.
    Membrane-bound pyrophosphatase of Thermotoga maritima requires sodium for activity (2005), Biochemistry, 44, 2088-2096 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.1.3.2 K+ enzyme exhibits an absolute requirement for Na+ but displays the highest activity in the presence of millimolar levels of both Na+ and K+. Two Na+ binding sites and one K+ binding site are involved in enzyme activation Thermotoga maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.1 expression in Escherichia coli Thermotoga maritima
7.1.3.2 expression in Escherichia coli Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.1 D190A the Na+ dose dependency of the D190A variant closely resembles that of the wild-type enzyme Thermotoga maritima
3.6.1.1 D703N the variant exhibits a much lower slope on the rate vs [Na+] plot than the wild-type enzyme at all of the K+ concentrations examined Thermotoga maritima
7.1.3.2 D190A the Na+ dose dependencies closely resemble those of the wild-type enzyme Thermotoga maritima
7.1.3.2 D190A/D703N activation by alkali cations is similar to the D703N variant, but the maximum is shifted to 2fold higher Na+ and K+ concentrations Thermotoga maritima
7.1.3.2 D703N only one Na+ binding site (K+-dependent) and one K+ binding site are involved in activation of the variant Thermotoga maritima

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.1 membrane bound to Thermotoga maritima 16020
-
7.1.3.2 membrane
-
 Thermotoga maritima 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 K+ the enzyme absolutely requires Na+ for activity, whereas both Na+ and K+ are needed for maximal activity Thermotoga maritima
3.6.1.1 Li+ Li+ can substitute for Na+ as activator. In the presence of K+, Li+ iss a less potent activator than Na+ Thermotoga maritima
3.6.1.1 Na+ the enzyme absolutely requires Na+ for activity, whereas both Na+ and K+ are needed for maximal activity Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Thermotoga maritima Q9S5X0
-
-
3.6.1.1 Thermotoga maritima DSM 3109 Q9S5X0
-
-
7.1.3.2 Thermotoga maritima Q9S5X0
-
-
7.1.3.2 Thermotoga maritima DSM 3109 Q9S5X0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.1
-
 Thermotoga maritima

Storage Stability

EC Number Storage Stability Organism
7.1.3.2 4°C, stable for at least 1 week Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O
-
 Thermotoga maritima 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O
-
 Thermotoga maritima DSM 3109 2 phosphate
-
 ?
7.1.3.2 diphosphate + H2O + Na+(side1)
-
 Thermotoga maritima H+ + Na+(side2) + 2 phosphate
-
 ?
7.1.3.2 diphosphate + H2O + Na+(side1)
-
 Thermotoga maritima DSM 3109 H+ + Na+(side2) + 2 phosphate
-
 ?
7.1.3.2 additional information enzyme does not catalyze oxygen exchange between phosphate and water. Solubilized HppA exhibits low but measurable PPi-synthesizing activity, which also requires Na+ but is inhibited by K+ Thermotoga maritima ?
-
-
7.1.3.2 additional information enzyme exhibits an absolute requirement for Na+ but displays the highest activity in the presence of millimolar levels of both Na+ and K+. Two Na+ binding sites and one K+ binding site are involved in enzyme activation. In the absence of K+, Li+ may substitute for Na+ Thermotoga maritima ?
-
-
7.1.3.2 additional information enzyme does not catalyze oxygen exchange between phosphate and water. Solubilized HppA exhibits low but measurable PPi-synthesizing activity, which also requires Na+ but is inhibited by K+ Thermotoga maritima DSM 3109 ?
-
-
7.1.3.2 additional information enzyme exhibits an absolute requirement for Na+ but displays the highest activity in the presence of millimolar levels of both Na+ and K+. Two Na+ binding sites and one K+ binding site are involved in enzyme activation. In the absence of K+, Li+ may substitute for Na+ Thermotoga maritima DSM 3109 ?
-
-

Subunits

EC Number Subunits Comment Organism
7.1.3.2 ? x * 66000, SDS-PAGE, x * 73000, calculated from sequence Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
3.6.1.1 Tm-PPase
-
 Thermotoga maritima
7.1.3.2 HppA
-
 Thermotoga maritima
7.1.3.2 K(+)-stimulated pyrophosphate-energized sodium pump
-
 Thermotoga maritima
7.1.3.2 TM_0174
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.1 40
-
 assay at Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.1 7.2
-
 assay at Thermotoga maritima