EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.1.27 | expression in Escherichia coli | Methylotuvimicrobium alcaliphilum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.1.27 | 40000 | - |
PAGE | Methylotuvimicrobium alcaliphilum |
5.3.1.27 | 70000 | - |
PAGE | Methylotuvimicrobium alcaliphilum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.27 | Methylotuvimicrobium alcaliphilum | G4T0K4 | - |
- |
5.3.1.27 | Methylotuvimicrobium alcaliphilum | G4T2U5 | hexulose-6-phosphate synthase, activity of EC 4.1.2.43, and isomerase | - |
5.3.1.27 | Methylotuvimicrobium alcaliphilum 20Z | G4T0K4 | - |
- |
5.3.1.27 | Methylotuvimicrobium alcaliphilum 20Z | G4T2U5 | hexulose-6-phosphate synthase, activity of EC 4.1.2.43, and isomerase | - |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.1.27 | dimer | 2 * 20000, SDS-PAGE, 2 * 19200, calculated from sequence | Methylotuvimicrobium alcaliphilum |
5.3.1.27 | dimer | 2 * 40000, SDS-PAGE, 2 * 41900, calculated from sequence | Methylotuvimicrobium alcaliphilum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.1.27 | Hpi1 | - |
Methylotuvimicrobium alcaliphilum |
5.3.1.27 | Hpsi2 | - |
Methylotuvimicrobium alcaliphilum |
5.3.1.27 | PHI | - |
Methylotuvimicrobium alcaliphilum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.3.1.27 | physiological function | 3-hexulose-6-phosphate synthase (HPS) and 6-phospho-3-hexulose isomerase (PHI) are encoded by the genes Hps and Phi and the fused gene Hps-Phi (Hpsi2). Both 3-hexulose-6-phosphate synthase plus 6-phospho-3-hexulose isomerase and the fused gene product catalyze formation of fructose 6-phosphate from formaldehyde and ribulose 5-phosphate with activities of 172 and 22 U/mg, respectively. the fusion protein Hpsi2 does not show isomerase activity, but the sequences corresponding to its HPS and PHI regions, when expressed separately, produce active enzymes | Methylotuvimicrobium alcaliphilum |
5.3.1.27 | physiological function | 3-hexulose-6-phosphate synthase (HPS) and 6-phospho-3-hexulose isomerase (PHI) are encoded by the genes Hps and Phi and the fused gene Hps-Phi. Both 3-hexulose-6-phosphate synthase plus 6-phospho-3-hexulose isomerase and the fused gene product catalyze formation of fructose 6-phosphate from formaldehyde and ribulose 5-phosphate with activities of 172 and 22 U/mg, respectively | Methylotuvimicrobium alcaliphilum |