Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kruse, I.; Maclean, A.E.; Hill, L.; Balk, J.
    Genetic dissection of cyclic pyranopterin monophosphate biosynthesis in plant mitochondria (2018), Biochem. J., 475, 495-509 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.22 gene CNX2, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes CNX2, i.e. 2065 genome-wide SNPs and 54 within the mapping interval of chromosome 2, viable allele of cnx2 in Arabidopsis thaliana Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
4.1.99.22 additional information generation of cnx2 mutants by T-DNA insertion, line cnx2-1, and point mutation, line cnx2-2. Mutation mapping by whole genome sequencing, phenotype analyses Arabidopsis thaliana
4.1.99.22 R88Q mutant xd22 has a mutation in CNX2 affecting the proximal FeS cluster loop. Complementation of the cnx2-2 phenotype by a genomic fragment including the promoter region from position -1224 and UTRs, amplified by PCR. Mutant cnx2-2 plants are transformed using Agrobacterium tumefaciens strain GV3101. The cnx2-2 mutation is not a knockout allele and growth is rescued by ammonia. Crosses between heterozygous cnx2-1 CNX2 and homozygous cnx2-2 plants give F1 offspring in two phenotype categories: seedlings with wild-type appearance corresponding to cnx2-1 CNX2 and small chlorotic seedlings that are genotyped as cnx2-1 cnx2-2 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.99.22 mitochondrion
-
 Arabidopsis thaliana 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.99.22 Fe2+ CNX2/MOCS1A is an FeS enzyme depending on two Fe4S4 clusters, which are assembled by the mitochondrial FeS cluster assembly pathway Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.22 GTP + S-adenosyl-L-methionine + reduced electron acceptor Arabidopsis thaliana
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor
-
 ?
4.1.99.22 GTP + S-adenosyl-L-methionine + reduced electron acceptor Arabidopsis thaliana Col-0
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.22 Arabidopsis thaliana Q39055
-
-
4.1.99.22 Arabidopsis thaliana Col-0 Q39055
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.22 GTP + S-adenosyl-L-methionine + reduced electron acceptor
-
 Arabidopsis thaliana (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor
-
 ?
4.1.99.22 GTP + S-adenosyl-L-methionine + reduced electron acceptor
-
 Arabidopsis thaliana Col-0 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.99.22 AT2G31955
-
 Arabidopsis thaliana
4.1.99.22 cnx2
-
 Arabidopsis thaliana
4.1.99.22 CNX2/MOCS1A
-
 Arabidopsis thaliana
4.1.99.22 MOCS1A
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.22 Fe-S center CNX2/MOCS1A is an FeS enzyme depending on two Fe4S4 clusters, which are assembled by the mitochondrial FeS cluster assembly pathway Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
4.1.99.22 malfunction cyclic pyranopterin monophosphate (cPMP) is strongly decreased in cnx2 mutants. Phenotype of T-DNA insertion mutant line cnx2-1, the cnx2-1 knockout is seedling-lethal but growth is rescued by ammonia, overview. Mutant xd22 has a mutation in CNX2 affecting the proximal FeS cluster loop. With hypoxanthine as a substrate, no activity is detectable in leaves from cnx2-2 plants. Nitrate reductase activity in leaf extract from cnx2-2 is 55% compared to wild-type values. Genetic complementation and strongly decreased activities of Moco enzymes show that the mutation in xd22 (R88Q) is located in CNX2, line cnx2-2, encoding the first step in Moco biosynthesis. The cnx2-2 mutation is not a knockout allele and growth is rescued by ammonia. Crosses between heterozygous cnx2-1 CNX2 and homozygous cnx2-2 plants give F1 offspring in two phenotype categories: seedlings with wild-type appearance corresponding to cnx2-1 CNX2 and small chlorotic seedlings that are genotyped as cnx2-1 cnx2-2 Arabidopsis thaliana
4.1.99.22 metabolism molybdenum cofactor (Moco) biosynthesis starts with the condensation of GTP into cyclic pyranopterin monophosphate (cPMP) by the consecutive action of GTP 3',8-cyclase and cPMP synthase, encoded by CNX2 and CNX3 in plants, respectively, pathways for FeS cluster assembly and Moco biosynthesis in Arabidopsis thaliana, overview Arabidopsis thaliana