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Literature summary extracted from

  • Wang, W.; Cai, X.; Han, N.; Han, W.; Sugahara, K.; Li, F.
    Sequencing of chondroitin sulfate oligosaccharides using a novel exolyase from a marine bacterium that degrades hyaluronan and chondroitin sulfate/dermatan sulfate (2017), Biochem. J., 474, 3831-3848 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.2.21 analysis enzyme combined with chodroitin sulfatase ABC, EC 4.2.2.4, can be applied for the sequencing of chondroitin sulfate hexa- and octasaccharides with complex structures Vibrio sp. FC509

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.21 expression in Escherichia coli Vibrio sp. FC509

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.21 soluble recombinant protein Vibrio sp. FC509
-
-

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.21 Vibrio sp. FC509 A0A077ERI8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.21 recombinant protein Vibrio sp. FC509

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.21 750
-
 substrate dermatan sulfate, pH 7.0, 30°C Vibrio sp. FC509
4.2.2.21 800
-
 substrate hyaluronan, pH 7.0, 30°C Vibrio sp. FC509
4.2.2.21 1210
-
 substrate chondroitin sulfate C, pH 7.0, 30°C Vibrio sp. FC509
4.2.2.21 1230
-
 substrate chondroitin sulfate A, pH 7.0, 30°C Vibrio sp. FC509
4.2.2.21 1350
-
 substrate chondroitin sulfate D, pH 7.0, 30°C Vibrio sp. FC509
4.2.2.21 1480
-
 substrate chondroitin sulfate E, pH 7.0, 30°C Vibrio sp. FC509

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.21 2-aminobenzamide-labeled chondroitin sulfate-C hexasaccharide
-
 Vibrio sp. FC509 unsaturated 2-aminobenzamide-labeled chondroitin sulfate-C disaccharide + ?
-
 ?
4.2.2.21 2-aminobenzamide-labeled chondroitin sulfate-C tetrasaccharide
-
 Vibrio sp. FC509 unsaturated 2-aminobenzamide-labeled chondroitin sulfate-C disaccharide + disaccharide
-
 ?
4.2.2.21 chondroitin sulfate A
-
 Vibrio sp. FC509 unsaturated chondroitin sulfate A disaccharides
-
 ?
4.2.2.21 chondroitin sulfate C
-
 Vibrio sp. FC509 unsaturated chondroitin sulfate C disaccharides
-
 ?
4.2.2.21 chondroitin sulfate D
-
 Vibrio sp. FC509 unsaturated chondroitin sulfate D disaccharides
-
 ?
4.2.2.21 chondroitin sulfate E
-
 Vibrio sp. FC509 unsaturated chondroitin sulfate E disaccharides
-
 ?
4.2.2.21 dermatan sulfate
-
 Vibrio sp. FC509 unsaturated dermatan disaccharides
-
 ?
4.2.2.21 hyaluronan
-
 Vibrio sp. FC509 unsaturated hyaluronan disaccharides
-
 ?
4.2.2.21 additional information no substrate: 2-aminobenzamide-labeled hyaluronan oligosaccharides, 2-aminobenzamide-labeled unsulfated chondroitin oligisaccharides, 2-aminobenzamide-labeled dermatan sulfate oligisaccharides Vibrio sp. FC509 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.21 ? x * 114700, calculated from sequence, x * 115000, SDS-PAGE Vibrio sp. FC509

Synonyms

EC Number Synonyms Comment Organism
4.2.2.21 HCLase
-
 Vibrio sp. FC509

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.21 30
-
-
 Vibrio sp. FC509

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.21 7
-
-
 Vibrio sp. FC509

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.21 6
-
 about 60% of maximum activity Vibrio sp. FC509

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.21 Vibrio sp. FC509 calculated from sequence
-
 5.2