EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.111 | expressed in Escherichia coli BL21(DE3) cells | Fischerella ambigua |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.111 | sitting drop vapor diffusion method, using 1.0 M sodium citrate, 0.1 M imidazole, pH 8.0 | Fischerella ambigua |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.146 | 3-[(E)-2-isocyanoethenyl]-1H-indole + geranyl diphosphate | Fischerella ambigua | preferred reaction | 2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3-[(Z)-2-isocyanoethenyl]-1H-indole + 3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3-[(Z)-2-isocyanoethenyl]-3H-indole | - |
? | |
2.5.1.146 | 3-[(E)-2-isocyanoethenyl]-1H-indole + geranyl diphosphate | Fischerella ambigua UTEX 1903 | preferred reaction | 2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3-[(Z)-2-isocyanoethenyl]-1H-indole + 3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3-[(Z)-2-isocyanoethenyl]-3H-indole | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.111 | Fischerella ambigua | A0A1J0AKI6 | - |
- |
2.5.1.111 | Fischerella ambigua UTEX 1903 | A0A1J0AKI6 | - |
- |
2.5.1.146 | Fischerella ambigua | V5TDZ4 | - |
- |
2.5.1.146 | Fischerella ambigua UTEX 1903 | V5TDZ4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.111 | Ni-NTA column chromatography | Fischerella ambigua |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.111 | dimethylallyl diphosphate + (2Z)-3-(1H-indol-3-yl)prop-2-enenitrile | dimethylallyl diphosphate is preferred over geranyl diphosphate | Fischerella ambigua | diphosphate + (2E)-3-[2-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]prop-2-enenitrile + (2E)-3-[2-(3-methylbut-2-en-1-yl)-3H-indol-3-yl]prop-2-enenitrile | - |
? | |
2.5.1.111 | dimethylallyl diphosphate + (2Z)-3-(1H-indol-3-yl)prop-2-enenitrile | dimethylallyl diphosphate is preferred over geranyl diphosphate | Fischerella ambigua UTEX 1903 | diphosphate + (2E)-3-[2-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]prop-2-enenitrile + (2E)-3-[2-(3-methylbut-2-en-1-yl)-3H-indol-3-yl]prop-2-enenitrile | - |
? | |
2.5.1.111 | dimethylallyl diphosphate + hapalindole U | - |
Fischerella ambigua | diphosphate + ambiguine H | - |
? | |
2.5.1.111 | dimethylallyl diphosphate + hapalindole U | - |
Fischerella ambigua UTEX 1903 | diphosphate + ambiguine H | - |
? | |
2.5.1.111 | geranyl diphosphate + (2Z)-3-(1H-indol-3-yl)prop-2-enenitrile | dimethylallyl diphosphate is preferred over geranyl diphosphate | Fischerella ambigua | diphosphate + (2Z)-3-(2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-1H-indol-3-yl)prop-2-enenitrile + (2E)-3-(2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3H-indol-3-yl)prop-2-enenitrile | - |
? | |
2.5.1.111 | geranyl diphosphate + (2Z)-3-(1H-indol-3-yl)prop-2-enenitrile | dimethylallyl diphosphate is preferred over geranyl diphosphate | Fischerella ambigua UTEX 1903 | diphosphate + (2Z)-3-(2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-1H-indol-3-yl)prop-2-enenitrile + (2E)-3-(2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3H-indol-3-yl)prop-2-enenitrile | - |
? | |
2.5.1.146 | 3-[(E)-2-isocyanoethenyl]-1H-indole + dimethylallyl diphosphate | - |
Fischerella ambigua | 3-[(E)-2-isocyanoethenyl]-2-(3-methylbut-2-en-1-yl)-1H-indole + 3-[(E)-2-isocyanoethenyl]-3-(3-methylbut-2-en-1-yl)-3H-indole | - |
? | |
2.5.1.146 | 3-[(E)-2-isocyanoethenyl]-1H-indole + dimethylallyl diphosphate | - |
Fischerella ambigua UTEX 1903 | 3-[(E)-2-isocyanoethenyl]-2-(3-methylbut-2-en-1-yl)-1H-indole + 3-[(E)-2-isocyanoethenyl]-3-(3-methylbut-2-en-1-yl)-3H-indole | - |
? | |
2.5.1.146 | 3-[(E)-2-isocyanoethenyl]-1H-indole + geranyl diphosphate | preferred reaction | Fischerella ambigua | 2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3-[(Z)-2-isocyanoethenyl]-1H-indole + 3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3-[(Z)-2-isocyanoethenyl]-3H-indole | - |
? | |
2.5.1.146 | 3-[(E)-2-isocyanoethenyl]-1H-indole + geranyl diphosphate | preferred reaction | Fischerella ambigua UTEX 1903 | 2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3-[(Z)-2-isocyanoethenyl]-1H-indole + 3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3-[(Z)-2-isocyanoethenyl]-3H-indole | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.111 | apT | - |
Fischerella ambigua |
2.5.1.111 | aromatic prenyltransferase | - |
Fischerella ambigua |
2.5.1.111 | FamD1 | - |
Fischerella ambigua |
2.5.1.146 | famD2 | - |
Fischerella ambigua |