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Literature summary extracted from

  • Yamamoto, K.; Higashiura, A.; Suzuki, M.; Aritake, K.; Urade, Y.; Nakagawa, A.
    Molecular structure of a prostaglandin D synthase requiring glutathione from the brown planthopper, Nilaparvata lugens (2017), Biochem. Biophys. Res. Commun., 492, 166-171 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.3.99.2 glutathione transferase
-
 Nilaparvata lugens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.2 expressed in Escherichia coli Rosetta (DE3) pLysS cells Nilaparvata lugens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.99.2 in complex with glutathione, sitting drop vapor diffusion method, using 0.1 M Tris-HCl, pH 8.0, 28% (w/v) PEG4000 Nilaparvata lugens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.99.2 0.32
-
 GSH at pH 8.0, temperature not specified in the publication Nilaparvata lugens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.99.2 Prostaglandin H2 Nilaparvata lugens
-
 Prostaglandin D2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.2 Nilaparvata lugens J9Q529 strain Izumo
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.99.2 DEAE-Sepharose column chromatography and Superdex 200 gel filtration Nilaparvata lugens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.2 1-chloro-2,4-dinitrobenzene + GSH
-
 Nilaparvata lugens ?
-
 ?
5.3.99.2 additional information the enzyme is inactive toward 1,2-epoxy-3-(4-nitrophenoxy)-propane, 4-nitrophenethyl bromide, 4-nitrobenzyl chloride, 4-hydroxynonenal, ethacrynic acid, and 4-nitrophenyl acetate Nilaparvata lugens ?
-
 ?
5.3.99.2 Prostaglandin H2
-
 Nilaparvata lugens Prostaglandin D2
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.99.2 homodimer 2 * 23000, SDS-PAGE Nilaparvata lugens

Synonyms

EC Number Synonyms Comment Organism
5.3.99.2 PGDS
-
 Nilaparvata lugens
5.3.99.2 Prostaglandin D synthase
-
 Nilaparvata lugens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.99.2 8
-
-
 Nilaparvata lugens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.99.2 1.7
-
 GSH at pH 8.0, temperature not specified in the publication Nilaparvata lugens