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Literature summary extracted from
- Pyruvate decarboxylase activity of the acetohydroxyacid synthase of Thermotoga maritima (2016), Biochem. Biophys. Rep., 7, 394-399 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.1.1 | expressed in Escherichia coli BL21(DE3) Rosetta 2 cells | Thermotoga maritima |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.1 | Mg2+ | dependent on | Thermotoga maritima |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.1 | pyruvate | Thermotoga maritima | - |
acetaldehyde + CO2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.1 | Thermotoga maritima | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.1.1 | DEAE column chromatography and HAP column chromatography | Thermotoga maritima |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.1 | pyruvate | - |
Thermotoga maritima | acetaldehyde + CO2 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.1.1 | acetohydroxyacid synthase | the enzyme also has approximately 10% pyruvate decarboxylase activity | Thermotoga maritima |
| 4.1.1.1 | AHAS | - |
Thermotoga maritima |
| 4.1.1.1 | PDC | - |
Thermotoga maritima |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.1 | 85 | - |
- |
Thermotoga maritima |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.1 | 4 | - |
about 40% of the activity is lost after 5 days incubation at 4°C (without stirring) | Thermotoga maritima |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.1 | 7 | - |
- |
Thermotoga maritima |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.1 | thiamine diphosphate | - |
Thermotoga maritima | |
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Release 2023.1 (January 2023)
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