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Literature summary extracted from

  • Kühner, M.; Haufschildt, K.; Neumann, A.; Storbeck, S.; Streif, J.; Layer, G.
    The alternative route to heme in the methanogenic archaeon Methanosarcina barkeri (2014), Archaea, 2014, 327637 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.98.6 expressed in Escherichia coli BL21(DE3) cells Methanosarcina barkeri
1.3.98.6 expression in Escherichia coli Methanosarcina barkeri
4.1.1.111 expressed in Escherichia coli BL21(DE3) cells Methanosarcina barkeri

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.111 additional information not inhibited by heme Methanosarcina barkeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.98.6 Fe2+ contains 8.5 mol iron per mol enzyme Methanosarcina barkeri
1.3.98.6 Iron presence of 8.5 mol iron per mol AhbD and 5.1 mol sulfide per mol AhbD Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.98.6 Fe-coproporphyrin III + 2 S-adenosyl-L-methionine Methanosarcina barkeri
-
 protoheme + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine
-
 ?
1.3.98.6 Fe-coproporphyrin III + 2 S-adenosyl-L-methionine Methanosarcina barkeri DSM 804
-
 protoheme + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine
-
 ?
1.3.98.6 Fe-coproporphyrin III + S-adenosyl-L-methionine Methanosarcina barkeri
-
 protoheme + CO2 + 5'-deoxyadenosine + L-methionine
-
 ?
4.1.1.111 siroheme Methanosarcina barkeri
-
 12,18-didecarboxysiroheme + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.98.6 Methanosarcina barkeri
-
-
-
1.3.98.6 Methanosarcina barkeri Q46CH7
-
-
1.3.98.6 Methanosarcina barkeri DSM 804 Q46CH7
-
-
4.1.1.111 Methanosarcina barkeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.98.6 Blue Sepharose column chromatography and Mono Q column chromatography Methanosarcina barkeri
4.1.1.111 Ni-NTA column chromatography and NAP-25 Sephadex gel filtration Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.98.6 Fe-coproporphyrin III + 2 S-adenosyl-L-methionine
-
 Methanosarcina barkeri protoheme + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine
-
 ?
1.3.98.6 Fe-coproporphyrin III + 2 S-adenosyl-L-methionine
-
 Methanosarcina barkeri DSM 804 protoheme + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine
-
 ?
1.3.98.6 Fe-coproporphyrin III + S-adenosyl-L-methionine
-
 Methanosarcina barkeri protoheme + CO2 + 5'-deoxyadenosine + L-methionine
-
 ?
4.1.1.111 siroheme
-
 Methanosarcina barkeri 12,18-didecarboxysiroheme + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.111 heterodimer
-
 Methanosarcina barkeri

Synonyms

EC Number Synonyms Comment Organism
1.3.98.6 ahbD
-
 Methanosarcina barkeri
1.3.98.6 Mbar_A1458
-
 Methanosarcina barkeri
4.1.1.111 AhbA subunit Methanosarcina barkeri
4.1.1.111 AhbB subunit Methanosarcina barkeri
4.1.1.111 sirohaem decarboxylase
-
 Methanosarcina barkeri

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.98.6 4Fe-4S-center presence of two [4Fe-4S] clusters Methanosarcina barkeri
1.3.98.6 S-adenosyl-L-methionine
-
 Methanosarcina barkeri
1.3.98.6 [4Fe-4S]-center the enzyme contains two [4Fe-4S] clusters Methanosarcina barkeri