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Literature summary extracted from

  • Kim, H.; Wang, J.; Lee, J.; Park, A.; Park, H.; Jeon, S.
    Biochemical and structural characterization of quinoprotein aldose sugar dehydrogenase from Thermus thermophilus HJ6 Mutational analysis of Tyr156 in the substrate-binding site (2016), Arch. Biochem. Biophys., 608, 20-26 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.5.2 analysis its high stability at high temperature makes this enzyme potentially useful for applications in biosensors or biofuel cells Thermus thermophilus
1.1.5.2 biofuel production its high stability at high temperature makes this enzyme potentially useful for applications in biosensors or biofuel cells Thermus thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.2 expression in Escherichia coli BL21, wild-type and mutant enzymes Y156A and Y156K Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.2 Y156A impaired activities and affinities for all substrates and completely loses the activity for alcohols. The catalytic efficiency of the Y156A mutant is lower than that of the Y156K mutant for most substrates Thermus thermophilus
1.1.5.2 Y156K impaired activities and affinities for all substrates and completely loses the activity for alcohols. The catalytic efficiency of the Y156A mutant is lower than that of the Y156K mutant for most substrates Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.2 0.000004
-
2-deoxy-D-glucose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.00002
-
D-glucosamine pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.00002
-
D-glucosamine pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.00002
-
D-glucosamine pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 0.00007
-
D-cellobiose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.00007
-
D-ribose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.00008
-
D-glucose 6-phosphate pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.00008
-
2-deoxy-D-glucose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.00009
-
xylitol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.00009
-
mannitol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.00011
-
D-galactose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.00013
-
maltotriose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.00013
-
D-mannose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.00013
-
D-ribose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.00014
-
D-xylose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.00014
-
D-mannose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.00014
-
L-arabinose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 0.00014
-
2-deoxy-D-glucose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 0.00015
-
methanol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.00016
-
L-arabinose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.00016
-
maltotriose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 0.00016
-
D-mannose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 0.00017
-
maltose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.00017
-
L-arabinose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.00018
-
ethanol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.00019
-
alpha-D-lactose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.0002
-
D-galactose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 0.00021
-
D-glucose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.00023
-
D-xylose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 0.00023
-
D-cellobiose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 0.00025
-
D-glucose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 0.00026
-
maltose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 0.0003
-
D-cellobiose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.00037
-
maltose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.00038
-
D-galactose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.00044
-
D-ribose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 0.00046
-
D-xylose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.00047
-
D-glucose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.0006
-
maltotriose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.00064
-
alpha-D-lactose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.00065
-
alpha-D-lactose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.2 37000
-
SDS-PAGE Thermus thermophilus
1.1.5.2 38871
-
calculated from sequence Thermus thermophilus
1.1.5.2 80000
-
gel filtration Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Thermus thermophilus I7A144
-
-
1.1.5.2 Thermus thermophilus HJ6 I7A144
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.2
-
Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 2-deoxy-D-glucose + phenazine methosulfate
-
Thermus thermophilus 2-deoxy-D-glucono-1,5-lactone + reduced phenazine methosulfate
-
?
1.1.5.2 2-deoxy-D-glucose + phenazine methosulfate
-
Thermus thermophilus HJ6 2-deoxy-D-glucono-1,5-lactone + reduced phenazine methosulfate
-
?
1.1.5.2 alpha-D-lactose + phenazine methosulfate
-
Thermus thermophilus alpha-D-lactono-1,5-lactone + reduced phenazine methosulfate
-
?
1.1.5.2 alpha-D-lactose + phenazine methosulfate
-
Thermus thermophilus HJ6 alpha-D-lactono-1,5-lactone + reduced phenazine methosulfate
-
?
1.1.5.2 D-cellobiose + phenazine methosulfate
-
Thermus thermophilus D-cellobiono-1,5-lactone + reduced phenazine methosulfate
-
?
1.1.5.2 D-galactose + phenazine methosulfate
-
Thermus thermophilus D-galactono-1,5-lactone + reduced phenazine methosulfate
-
?
1.1.5.2 D-glucosamino-1,5-lactone + reduced phenazine methosulfate
-
Thermus thermophilus D-glucosamine + reduced phenazine methosulfate
-
?
1.1.5.2 D-glucose + phenazine methosulfate
-
Thermus thermophilus D-glucono-1,5-lactone + reduced phenazine methosulfate
-
?
1.1.5.2 D-glucose + phenazine methosulfate
-
Thermus thermophilus HJ6 D-glucono-1,5-lactone + reduced phenazine methosulfate
-
?
1.1.5.2 D-glucose 6-phosphate + phenazine methosulfate
-
Thermus thermophilus 6-phospho-D-glucono-1,5-lactone + reduced phenazine methosulfate
-
?
1.1.5.2 D-mannose + phenazine methosulfate
-
Thermus thermophilus D-mannono-1,5-lactone + reduced phenazine methosulfate
-
?
1.1.5.2 D-ribose + phenazine methosulfate
-
Thermus thermophilus D-ribono-1,4-lactone + reduced phenazine methosulfate
-
?
1.1.5.2 D-xylose + phenazine methosulfate
-
Thermus thermophilus D-xylono-1,5-lactone + reduced phenazine methosulfate
-
?
1.1.5.2 ethanol + phenazine methosulfate
-
Thermus thermophilus acetaldehyde + reduced phenazine methosulfate
-
?
1.1.5.2 ethanol + phenazine methosulfate
-
Thermus thermophilus HJ6 acetaldehyde + reduced phenazine methosulfate
-
?
1.1.5.2 L-arabinose + phenazine methosulfate
-
Thermus thermophilus D-arabinono-1,5-lactone + reduced phenazine methosulfate
-
?
1.1.5.2 maltose + phenazine methosulfate
-
Thermus thermophilus D-maltono-1,5-lactone + reduced phenazine methosulfate
-
?
1.1.5.2 maltotriose + phenazine methosulfate
-
Thermus thermophilus D-maltotriono-1,5-lactone + reduced phenazine methosulfate
-
?
1.1.5.2 mannitol + phenazine methosulfate
-
Thermus thermophilus ?
-
?
1.1.5.2 methanol + phenazine methosulfate
-
Thermus thermophilus formaldehyde + reduced phenazine methosulfate
-
?
1.1.5.2 methanol + phenazine methosulfate
-
Thermus thermophilus HJ6 formaldehyde + reduced phenazine methosulfate
-
?
1.1.5.2 xylitol + phenazine methosulfate
-
Thermus thermophilus ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.5.2 homodimer 2 * 37000, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.1.5.2 quinoprotein aldose sugar dehydrogenase
-
Thermus thermophilus
1.1.5.2 Tt_ASD
-
Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.2 70
-
-
Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.5.2 40
-
about 50% of maximal activity Thermus thermophilus
1.1.5.2 60 80 the recombinant enzyme shows more than 90% activity within the range of 60-80°C Thermus thermophilus
1.1.5.2 85
-
about 50% of maximal activity Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.5.2 85
-
half-life of apoenzyme: about 25 min. Activity of the pyrroloquinoline quinone-bound holoenzyme is not lost after incubation at 85°C for 100 min Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.5.2 15
-
xylitol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 38
-
mannitol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 69
-
2-deoxy-D-glucose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 76
-
methanol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 104
-
D-glucosamine pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 117
-
D-glucosamine pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 123
-
ethanol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 128
-
2-deoxy-D-glucose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 142
-
L-arabinose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 154
-
L-arabinose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 160
-
D-galactose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 160
-
D-cellobiose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 170
-
maltose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 181
-
D-ribose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 186
-
maltose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 186
-
D-cellobiose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 192
-
D-glucose 6-phosphate pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 193
-
D-galactose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 197
-
D-glucosamine pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 204
-
D-xylose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 225
-
alpha-D-lactose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 260
-
D-ribose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 261
-
D-mannose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 265
-
D-mannose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 282
-
alpha-D-lactose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 305
-
D-xylose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 316
-
D-glucose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 339
-
D-glucose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 363
-
maltotriose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 715
-
D-ribose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 772
-
maltotriose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 822
-
maltotriose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 823
-
D-galactose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 1022
-
maltose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 1042
-
D-cellobiose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 1054
-
D-glucose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 1141
-
2-deoxy-D-glucose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 1627
-
D-mannose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 1681
-
D-xylose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 1755
-
L-arabinose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 1953
-
alpha-D-lactose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.2 7 7.5
-
Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.5.2 6 9 pH 6.0: about 50% of maximal activity, pH 9.0: about 55% of maximal activity Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.2 pyrroloquinoline quinone prosthetic group Thermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.5.2 0.173
-
xylitol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.343
-
alpha-D-lactose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 0.414
-
mannitol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.417
-
D-galactose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.439
-
alpha-D-lactose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.5
-
maltose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.506
-
methanol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.527
-
D-cellobiose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.654
-
maltose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 0.68
-
ethanol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 0.699
-
D-xylose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.719
-
D-glucose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.796
-
L-arabinose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.808
-
D-cellobiose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 0.872
-
D-xylose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 0.903
-
2-deoxy-D-glucose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 0.921
-
2-deoxy-D-glucose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 0.952
-
D-galactose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 1.079
-
L-arabinose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 1.287
-
D-glucose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 1.366
-
maltotriose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 1.61
-
D-mannose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 1.631
-
D-ribose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 1.858
-
D-mannose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 2.062
-
D-ribose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 2.283
-
maltotriose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 2.543
-
D-glucose 6-phosphate pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 2.724
-
D-ribose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 5.021
-
D-glucose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 5.727
-
D-glucosamine pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.1.5.2 6.012
-
maltose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 6.16
-
maltotriose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 7.488
-
D-galactose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 8.696
-
D-glucosamine pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.1.5.2 10.28
-
alpha-D-lactose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 10.972
-
L-arabinose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 12.011
-
D-xylose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 12.518
-
D-mannose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 12.989
-
D-glucosamine pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 14.893
-
D-cellobiose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1.1.5.2 29.483
-
2-deoxy-D-glucose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus