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Literature summary extracted from

  • Martinkova, L.; Vesela, A.B.; Rinagelova, A.; Chmatal, M.
    Cyanide hydratases and cyanide dihydratases emerging tools in the biodegradation and biodetection of cyanide (2015), Appl. Microbiol. Biotechnol., 99, 8875-8882 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.5.1 analysis tools in the biodegradation and biodetection of cyanide Bacillus pumilus
4.2.1.66 analysis tools in the biodegradation and biodetection of cyanide Fusarium lateritium

Protein Variants

EC Number Protein Variants Comment Organism
3.5.5.1 E327G/Q86R/E96G/D254E/E35K/Q322R/E327G/Q86R/E6G/D254E/E327G mutamt enzyme shows activity at pH 10. The wild-type enzyme exhibits the optimum activity at pH 8 and is not able to hydrolyze HCN at pH higher than 9. However, cyanide wastes should be maintained at alkaline pH to prevent HCN release. Therefore, the CynD from Bacillus pumilus was engineered for an increased activity under these conditions (pH 9-10), and several mutants were shown to meet this requirement Bacillus pumilus
4.2.1.66 F170L no activity Fusarium lateritium
4.2.1.66 K136R/D275E/V281A/M302S mutant enzyme shows lower activity Fusarium lateritium
4.2.1.66 T12Q/S13A mutant enzyme shows lower activity Fusarium lateritium

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Bacillus pumilus B3GNT7
-
-
4.2.1.66 Fusarium lateritium P32963
-
-

Synonyms

EC Number Synonyms Comment Organism
3.5.5.1 CynD
-
 Bacillus pumilus
4.2.1.66 CHT
-
 Fusarium lateritium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 8
-
 wild-type enzyme Bacillus pumilus