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Literature summary extracted from
- Identification and distribution of cellobiose 2-epimerase genes by a PCR-based metagenomic approach (2015), Appl. Microbiol. Biotechnol., 99, 4287-4295 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.3.11 | gene md1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression in Escherichia coli | uncultured bacterium |
| 5.1.3.11 | gene md2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression in Escherichia coli | uncultured bacterium |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.3.11 | 3.92 | - |
cellobiose | recombinant enzyme, pH 7.5, 25°C | uncultured bacterium |  |
| 5.1.3.11 | 10.8 | - |
cellobiose | recombinant enzyme, pH 7.5, 30°C | uncultured bacterium | |
| 5.1.3.11 | 21.8 | - |
mannobiose | recombinant enzyme, pH 7.5, 30°C | uncultured bacterium | |
| 5.1.3.11 | 25.1 | - |
mannobiose | recombinant enzyme, pH 7.5, 25°C | uncultured bacterium | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.11 | cellobiose | uncultured bacterium | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.3.11 | uncultured bacterium | A0A0A1H8D7 | - |
- |
| 5.1.3.11 | uncultured bacterium | A0A0A1HAT7 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.3.11 | recombinant enzyme from Escherichia coli | uncultured bacterium |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.11 | 7.7 | - |
purified recombinant enzyme, pH 7.5, 25°C, substrate epilactose | uncultured bacterium |
| 5.1.3.11 | 9.06 | - |
purified recombinant enzyme, pH 7.5, 25°C, substrate lactose | uncultured bacterium |
| 5.1.3.11 | 10.4 | - |
purified recombinant enzyme, pH 7.5, 30°C, substrate lactose | uncultured bacterium |
| 5.1.3.11 | 21.1 | - |
purified recombinant enzyme, pH 7.5, 30°C, substrate epilactose | uncultured bacterium |
| 5.1.3.11 | 62.3 | - |
purified recombinant enzyme, pH 7.5, 25°C, substrate cellobiose | uncultured bacterium |
| 5.1.3.11 | 71.6 | - |
purified recombinant enzyme, pH 7.5, 30°C, substrate cellobiose | uncultured bacterium |
| 5.1.3.11 | 97.5 | - |
purified recombinant enzyme, pH 7.5, 30°C, substrate beta-1,4-mannobiose | uncultured bacterium |
| 5.1.3.11 | 201 | - |
purified recombinant enzyme, pH 7.5, 25°C, substrate beta-1,4-mannobiose | uncultured bacterium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.11 | cellobiose | - |
uncultured bacterium | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| 5.1.3.11 | cellobiose | (beta1,4) | uncultured bacterium | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| 5.1.3.11 | cellobiose | (beta1,4), best substrate of md2 | uncultured bacterium | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| 5.1.3.11 | epilactose | (beta1,4) | uncultured bacterium | lactose | - |
r | |
| 5.1.3.11 | lactose | (beta1,4) | uncultured bacterium | epilactose | - |
r | |
| 5.1.3.11 | mannobiose | (beta1,4) | uncultured bacterium | 4-O-beta-D-mannopyranosyl-D-glucose | - |
r | |
| 5.1.3.11 | mannobiose | (beta1,4), best substrate of md1 | uncultured bacterium | 4-O-beta-D-mannopyranosyl-D-glucose | - |
r | |
| 5.1.3.11 | additional information | substrate specificitiy of mD1 toward beta-linked disaccharides, overview. The enzyme is active on mannobiose (beta-1,4), cellobiose (beta-1,4), lactose (beta-1,4), and epilactose (beta-1,4), but not on laminaribiose (beta-1,3), gentiobiose (beta-1,6), and xylobiose (beta-1,4). No epimerization activity toward monosaccharides such as mannose, glucose, xylose, galactose, fructose, and GlcNAc and alpha-linked disaccharides such as trehalose (alpha-1,1), kojibiose (alpha-1,2), nigerose (alpha-1,3), maltose (alpha-1,4), sucrose (alpha-1,4), and melibiose (alpha-1,6). Enzyme mD1 favors mannobiose over cellobiose for catalysis | uncultured bacterium | ? | - |
? | |
| 5.1.3.11 | additional information | substrate specificitiy of mD1 toward beta-linked disaccharides, overview. The enzyme is active on mannobiose (beta-1,4), cellobiose (beta-1,4), lactose (beta-1,4), and epilactose (beta-1,4), but not on laminaribiose (beta-1,3), gentiobiose (beta-1,6), and xylobiose (beta-1,4). No epimerization activity toward monosaccharides such as mannose, glucose, xylose, galactose, fructose, and GlcNAc and alpha-linked disaccharides such as trehalose (alpha-1,1), kojibiose (alpha-1,2), nigerose (alpha-1,3), maltose (alpha-1,4), sucrose (alpha-1,4), and melibiose (alpha-1,6). Enzyme mD2 favors mannobiose over cellobiose for catalysis | uncultured bacterium | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.3.11 | ? | x * 44800, recombinant enzyme, SDS-PAGE | uncultured bacterium |
| 5.1.3.11 | ? | x * 46600, recombinant enzyme, SDS-PAGE | uncultured bacterium |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.1.3.11 | cellobiose 2-epimerase | - |
uncultured bacterium |
| 5.1.3.11 | md1 | - |
uncultured bacterium |
| 5.1.3.11 | md2 | - |
uncultured bacterium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.11 | 25 | - |
- |
uncultured bacterium |
| 5.1.3.11 | 30 | - |
- |
uncultured bacterium |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.3.11 | 109 | - |
cellobiose | recombinant enzyme, pH 7.5, 30°C | uncultured bacterium | |
| 5.1.3.11 | 113 | - |
mannobiose | recombinant enzyme, pH 7.5, 25°C | uncultured bacterium | |
| 5.1.3.11 | 137 | - |
mannobiose | recombinant enzyme, pH 7.5, 30°C | uncultured bacterium | |
| 5.1.3.11 | 166 | - |
cellobiose | recombinant enzyme, pH 7.5, 25°C | uncultured bacterium | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.11 | 7.5 | - |
- |
uncultured bacterium |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.1.3.11 | evolution | the cluster of CE and CE-like proteins is functionally and evolutionarily separated from that of N-acetyl-D-glucosamine 2-epimerase (AGE) and AGE-like proteins, phylogenetic analysis | uncultured bacterium |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.3.11 | 4.5 | - |
mannobiose | recombinant enzyme, pH 7.5, 25°C | uncultured bacterium | |
| 5.1.3.11 | 6.33 | - |
mannobiose | recombinant enzyme, pH 7.5, 30°C | uncultured bacterium | |
| 5.1.3.11 | 11 | - |
cellobiose | recombinant enzyme, pH 7.5, 30°C | uncultured bacterium | |
| 5.1.3.11 | 42.8 | - |
cellobiose | recombinant enzyme, pH 7.5, 25°C | uncultured bacterium | |
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