Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, B.J.; Hong, S.H.; Shin, K.C.; Jo, Y.S.; Oh, D.K.
    Characterization of a F280N variant of L-arabinose isomerase from Geobacillus thermodenitrificans identified as a D-galactose isomerase (2014), Appl. Microbiol. Biotechnol., 98, 9271-9281 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.4
-
 Geobacillus thermodenitrificans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.4 homology modeling of mutant C450S/N475K Geobacillus thermodenitrificans

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.4 C450S/N475K mutant catalyzes the isomerization of D-galactose to D-tagatose Geobacillus thermodenitrificans
5.3.1.4 F280N/C450S/N475K mutat catalyzes the isomerization of D-galactose to D-tagatose Geobacillus thermodenitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.4 1.328
-
 D-galactose mutant F280N/C450S/N475K, pH 8, 55°C Geobacillus thermodenitrificans
5.3.1.4 106
-
 L-arabinose mutant C450S/N475K, pH 8, 55°C Geobacillus thermodenitrificans
5.3.1.4 334
-
 L-arabinose mutant F280N/C450S/N475K, pH 8, 55°C Geobacillus thermodenitrificans
5.3.1.4 339
-
 D-galactose mutant C450S/N475K, pH 8, 55°C Geobacillus thermodenitrificans

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Geobacillus thermodenitrificans Q6W9D4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 D-galactose
-
 Geobacillus thermodenitrificans D-tagatose
-
 ?
5.3.1.4 L-arabinose
-
 Geobacillus thermodenitrificans L-ribulose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.4 55
-
 mutant F280N/C450S/N475K Geobacillus thermodenitrificans
5.3.1.4 70
-
 mutant C450S/N475K Geobacillus thermodenitrificans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.4 65
-
 mutant C450S/N475K, half-life 728 min Geobacillus thermodenitrificans
5.3.1.4 65
-
 mutant F280N/C450S/N475K, half-life 108 min Geobacillus thermodenitrificans
5.3.1.4 75
-
 mutant C450S/N475K, half-life 47.4 min Geobacillus thermodenitrificans
5.3.1.4 75
-
 mutant F280N/C450S/N475K, half-life 13.3 min Geobacillus thermodenitrificans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.4 12.8
-
 L-arabinose mutant F280N/C450S/N475K, pH 8, 55°C Geobacillus thermodenitrificans
5.3.1.4 17.4
-
 D-galactose mutant C450S/N475K, pH 8, 55°C Geobacillus thermodenitrificans
5.3.1.4 104.1
-
 D-galactose mutant F280N/C450S/N475K, pH 8, 55°C Geobacillus thermodenitrificans
5.3.1.4 240.6
-
 L-arabinose mutant C450S/N475K, pH 8, 55°C Geobacillus thermodenitrificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 8
-
 mutant F280N/C450S/N475K Geobacillus thermodenitrificans
5.3.1.4 8.5
-
 mutant C450S/N475K Geobacillus thermodenitrificans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.4 0.04
-
 L-arabinose mutant F280N/C450S/N475K, pH 8, 55°C Geobacillus thermodenitrificans
5.3.1.4 0.05
-
 D-galactose mutant C450S/N475K, pH 8, 55°C Geobacillus thermodenitrificans
5.3.1.4 0.08
-
 D-galactose mutant F280N/C450S/N475K, pH 8, 55°C Geobacillus thermodenitrificans
5.3.1.4 2.27
-
 L-arabinose mutant C450S/N475K, pH 8, 55°C Geobacillus thermodenitrificans