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Literature summary extracted from

  • Jia, M.; Mu, W.; Chu, F.; Zhang, X.; Jiang, B.; Zhou, L.; Zhang, T.
    A D-psicose 3-epimerase with neutral pH optimum from Clostridium bolteae for D-psicose production Cloning, expression, purification, and characterization (2014), Appl. Microbiol. Biotechnol., 98, 717-725 .
    View publication on PubMed

Application

EC Number Application Comment Organism
5.1.3.30 synthesis the enzyme is a potential D-psicose producer for industrial production Enterocloster bolteae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.30 gene CLOBOL_00069, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli Enterocloster bolteae

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.30 additional information enzyme overexpression for heterologous production of D-psicose from D-fructose, under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31. For high production of D-psicose, 216 g/L D-psicose are produced with 28.8% turnover yield at pH 6.5 and 55°C. The recombinant DPEase exhibits weak acid stability and thermostability and has a high affinity and turnover for the substrate D-fructose Enterocloster bolteae

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.30 Cu2+
-
Enterocloster bolteae
5.1.3.30 EDTA complete inhibition Enterocloster bolteae
5.1.3.30 Mg2+
-
Enterocloster bolteae
5.1.3.30 Zn2+
-
Enterocloster bolteae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.30 27.4
-
D-psicose pH 7.0, 55°C, recombinant enzyme Enterocloster bolteae
5.1.3.30 59.8
-
D-fructose pH 7.0, 55°C, recombinant enzyme Enterocloster bolteae
5.1.3.30 339
-
D-tagatose pH 7.0, 55°C, recombinant enzyme Enterocloster bolteae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.30 Co2+ activates most highly at 0.5 mM, 80% of maximal activation at 1-5 mM, best divalent cation, dependent on Enterocloster bolteae
5.1.3.30 Mn2+ activates Enterocloster bolteae
5.1.3.30 additional information the CB-DPEase is a metalloprotein, that is inactive in absence of divalent metal ions Enterocloster bolteae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.30 D-psicose Enterocloster bolteae
-
D-fructose
-
r
5.1.3.30 D-psicose Enterocloster bolteae ATCC BAA-613
-
D-fructose
-
r

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.30 Enterocloster bolteae A8RG82
-
-
5.1.3.30 Enterocloster bolteae ATCC BAA-613 A8RG82
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.30 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity Enterocloster bolteae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.30 D-fructose 66.4% activity compared to the activity with D-psicose Enterocloster bolteae D-psicose
-
r
5.1.3.30 D-fructose 66.4% activity compared to the activity with D-psicose Enterocloster bolteae ATCC BAA-613 D-psicose
-
r
5.1.3.30 D-psicose
-
Enterocloster bolteae D-fructose
-
r
5.1.3.30 D-psicose i.e. D-ribo-2-hexulose or D-allulose, best substrate Enterocloster bolteae D-fructose
-
r
5.1.3.30 D-psicose
-
Enterocloster bolteae ATCC BAA-613 D-fructose
-
r
5.1.3.30 D-psicose i.e. D-ribo-2-hexulose or D-allulose, best substrate Enterocloster bolteae ATCC BAA-613 D-fructose
-
r
5.1.3.30 D-sorbose 1.2% activity compared to the activity with D-psicose Enterocloster bolteae D-tagatose
-
r
5.1.3.30 D-sorbose 1.2% activity compared to the activity with D-psicose Enterocloster bolteae ATCC BAA-613 D-tagatose
-
r
5.1.3.30 D-tagatose 23.2% activity compared to the activity with D-psicose Enterocloster bolteae D-sorbose
-
r
5.1.3.30 D-tagatose 23.2% activity compared to the activity with D-psicose Enterocloster bolteae ATCC BAA-613 D-sorbose
-
r

Subunits

EC Number Subunits Comment Organism
5.1.3.30 ? x * 33015, sequence calculation Enterocloster bolteae

Synonyms

EC Number Synonyms Comment Organism
5.1.3.30 CB-DPEase
-
Enterocloster bolteae
5.1.3.30 CLOBOL_00069
-
Enterocloster bolteae
5.1.3.30 DPEase
-
Enterocloster bolteae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.30 55
-
recombinant enzyme Enterocloster bolteae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.3.30 55
-
purified recombinant enzyme, half-life is 156 min Enterocloster bolteae
5.1.3.30 100
-
purified recombinant enzyme, 10 min, inactivation Enterocloster bolteae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.30 4.7
-
D-tagatose pH 7.0, 55°C, recombinant enzyme Enterocloster bolteae
5.1.3.30 49
-
D-psicose pH 7.0, 55°C, recombinant enzyme Enterocloster bolteae
5.1.3.30 59
-
D-fructose pH 7.0, 55°C, recombinant enzyme Enterocloster bolteae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.30 7
-
recombinant enzyme Enterocloster bolteae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.3.30 5 9 recombinant enzyme, activity range, profile overview Enterocloster bolteae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.3.30 5 9 purified recombinant enzyme, 80-100% of maximal activity within this range Enterocloster bolteae
5.1.3.30 7
-
purified recombinant enzyme, most stable at Enterocloster bolteae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.1.3.30 Enterocloster bolteae sequence calculation
-
5.02

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1.3.30 0.014
-
D-tagatose pH 7.0, 55°C, recombinant enzyme Enterocloster bolteae
5.1.3.30 0.99
-
D-fructose pH 7.0, 55°C, recombinant enzyme Enterocloster bolteae
5.1.3.30 1.78
-
D-psicose pH 7.0, 55°C, recombinant enzyme Enterocloster bolteae