EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.9 | Y97A | specific Glc-1-P UTase activity of the mutant enzyme is 2.3fold lower than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
2.7.7.9 | Y97C | specific Glc-1-P UTase activity of the mutant enzyme is 1.9fold lower than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
2.7.7.9 | Y97D | specific Glc-1-P UTase activity of the mutant enzyme is 6.2fold lower than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
2.7.7.9 | Y97E | mutant enzyme shows mutant enzyme shows no activity | Sulfurisphaera tokodaii |
2.7.7.9 | Y97F | specific Glc-1-P UTase activity of the mutant enzyme is 1.2fold lower than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
2.7.7.9 | Y97G | specific Glc-1-P UTase activity of the mutant enzyme is 4.2fold lower than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
2.7.7.9 | Y97H | specific Glc-1-P UTase activity of the mutant enzyme is 2.6fold higher than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
2.7.7.9 | Y97I | mutant enzyme shows no activity | Sulfurisphaera tokodaii |
2.7.7.9 | Y97K | specific Glc-1-P UTase activity of the mutant enzyme is 3.7fold lower than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
2.7.7.9 | Y97L | specific Glc-1-P UTase activity of the mutant enzyme is 2.4fold higher than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
2.7.7.9 | Y97M | specific Glc-1-P UTase activity of the mutant enzyme is 2.8fold higher than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
2.7.7.9 | Y97N | specific Glc-1-P UTase activity of the mutant enzyme is 4.1fold higher than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
2.7.7.9 | Y97Q | specific Glc-1-P UTase activity of the mutant enzyme is 1.04fold higher than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
2.7.7.9 | Y97R | mutant enzyme shows no activity | Sulfurisphaera tokodaii |
2.7.7.9 | Y97S | specific Glc-1-P UTase activity of the mutant enzyme is 1.1fold higher than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
2.7.7.9 | Y97T | specific Glc-1-P UTase activity of the mutant enzyme is 4fold lower than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
2.7.7.9 | Y97V | specific Glc-1-P UTase activity of the mutant enzyme is 3.34fold lower than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
2.7.7.9 | Y97W | mutant enzyme shows no activity | Sulfurisphaera tokodaii |
2.7.7.23 | G9A/K147A | specific GlcNAc-1-P UTase activity of the mutant enzyme is 71.8fold lower compared to specific activity of the wild-type enzyme | Sulfurisphaera tokodaii |
2.7.7.23 | G9A/T80A | specific GlcNAc-1-P UTase activity of the mutant enzyme is 35fold lower compared to specific activity of the wild-type enzyme | Sulfurisphaera tokodaii |
2.7.7.23 | G9A/Y97A | no activity | Sulfurisphaera tokodaii |
2.7.7.23 | G9A/Y97F | specific GlcNAc-1-P UTase activity of the mutant enzyme is 14.5fold lower compared to specific activity of the wild-type enzyme | Sulfurisphaera tokodaii |
2.7.7.23 | T80A/K147A | specific GlcNAc-1-P UTase activity of the mutant enzyme is 11.09fold lower compared to specific activity of the wild-type enzyme | Sulfurisphaera tokodaii |
2.7.7.23 | T80A/Y97A | specific GlcNAc-1-P UTase activity of the mutant enzyme is 31.4fold lower compared to specific activity of the wild-type enzyme | Sulfurisphaera tokodaii |
2.7.7.23 | T80A/Y97F | specific GlcNAc-1-P UTase activity of the mutant enzyme is 25.1fold lower compared to specific activity of the wild-type enzyme | Sulfurisphaera tokodaii |
2.7.7.23 | Y103A | mutant protein shows a 24% increase in activity compared to that of the wild-type enzyme | Escherichia coli |
2.7.7.23 | Y103N | mutant protein shows a 32% increase in activity compared to that of the wild-type enzyme | Escherichia coli |
2.7.7.23 | Y103V | mutant protein shows a 30% decrease in activity compared to that of the wild-type enzyme | Escherichia coli |
2.7.7.23 | Y97A | 1.82fold increase in GlcNAc-1-P UTase activity compared to that of the wild-type enzyme | Sulfurisphaera tokodaii |
2.7.7.23 | Y97A/K147A | specific GlcNAc-1-P UTase activity of the mutant enzyme is 11.9fold lower compared to specific activity of the wild-type enzyme | Sulfurisphaera tokodaii |
2.7.7.23 | Y97F/K147A | specific GlcNAc-1-P UTaseactivity of the mutant enzyme is 5.7fold lower compared to specific activity of the wild-type enzyme | Sulfurisphaera tokodaii |
2.7.7.23 | Y97N | 4.45fold increase in GlcNAc-1-P UTase activity compared to that of the wild-type enzyme | Sulfurisphaera tokodaii |
2.7.7.23 | Y97V | 3.56fold increase in GlcNAc-1-P UTase activity compared to that of the wild-type enzyme | Sulfurisphaera tokodaii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.9 | 1.23 | - |
alpha-D-glucose 1-phosphate | wild-type enzyme, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.9 | 1.74 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y97M, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.9 | 2.5 | 3 | alpha-D-glucose 1-phosphate | mutant enzyme Y97N, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.9 | 3.28 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y97V, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.23 | 0.068 | - |
N-acetyl-alpha-D-glucosamine 1-phosphate | wild-type enzyme, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.23 | 0.097 | - |
N-acetyl-alpha-D-glucosamine 1-phosphate | mutant enzyme Y97M, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.23 | 0.147 | - |
N-acetyl-alpha-D-glucosamine 1-phosphate | mutant enzyme Y97N, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.23 | 0.861 | - |
N-acetyl-alpha-D-glucosamine 1-phosphate | mutant enzyme Y97V, pH 7.5, 80°C | Sulfurisphaera tokodaii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.9 | Sulfurisphaera tokodaii | Q975F9 | - |
- |
2.7.7.9 | Sulfurisphaera tokodaii DSM 16993 | Q975F9 | - |
- |
2.7.7.23 | Escherichia coli | P0ACC7 | - |
- |
2.7.7.23 | Escherichia coli K12 | P0ACC7 | - |
- |
2.7.7.23 | Sulfurisphaera tokodaii | Q975F9 | - |
- |
2.7.7.23 | Sulfurisphaera tokodaii DSM 16993 | Q975F9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.9 | - |
Sulfurisphaera tokodaii |
2.7.7.23 | - |
Escherichia coli |
2.7.7.23 | - |
Sulfurisphaera tokodaii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.9 | UTP + alpha-D-glucose 1-phosphate | - |
Sulfurisphaera tokodaii | diphosphate + UDP-alpha-D-glucose | - |
? | |
2.7.7.9 | UTP + alpha-D-glucose 1-phosphate | - |
Sulfurisphaera tokodaii DSM 16993 | diphosphate + UDP-alpha-D-glucose | - |
? | |
2.7.7.23 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
Escherichia coli | diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? | |
2.7.7.23 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
Sulfurisphaera tokodaii | diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? | |
2.7.7.23 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
Sulfurisphaera tokodaii DSM 16993 | diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? | |
2.7.7.23 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
Escherichia coli K12 | diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.9 | Glc-1-P UTase | - |
Sulfurisphaera tokodaii |
2.7.7.9 | ST0452 | - |
Sulfurisphaera tokodaii |
2.7.7.23 | EcGlmU | - |
Escherichia coli |
2.7.7.23 | GlcNAc-1-P UTase | - |
Sulfurisphaera tokodaii |
2.7.7.23 | ST0452 | - |
Sulfurisphaera tokodaii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.9 | 80 | - |
assay at | Sulfurisphaera tokodaii |
2.7.7.23 | 37 | - |
assay at | Escherichia coli |
2.7.7.23 | 80 | - |
assay at | Sulfurisphaera tokodaii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.9 | 2.08 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y97V, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.9 | 2.97 | - |
alpha-D-glucose 1-phosphate | wild-type enzyme, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.9 | 14.97 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y97M, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.9 | 38.15 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y97N, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.23 | 9.97 | - |
N-acetyl-alpha-D-glucosamine 1-phosphate | wild-type enzyme, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.23 | 10.9 | - |
N-acetyl-alpha-D-glucosamine 1-phosphate | mutant enzyme Y97M, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.23 | 48.95 | - |
N-acetyl-alpha-D-glucosamine 1-phosphate | mutant enzyme Y97V, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.23 | 49.75 | - |
N-acetyl-alpha-D-glucosamine 1-phosphate | mutant enzyme Y97N, pH 7.5, 80°C | Sulfurisphaera tokodaii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.9 | 7.5 | - |
assay atz | Sulfurisphaera tokodaii |
2.7.7.23 | 7.5 | - |
assay at | Sulfurisphaera tokodaii |
2.7.7.23 | 7.5 | - |
assay | Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.9 | 0.63 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y97V, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.9 | 2.41 | - |
alpha-D-glucose 1-phosphate | wild-type enzyme, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.9 | 8.6 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y97M, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.9 | 15.08 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y97N, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.23 | 56.9 | - |
N-acetyl-alpha-D-glucosamine 1-phosphate | mutant enzyme Y97V, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.23 | 112.4 | - |
N-acetyl-alpha-D-glucosamine 1-phosphate | mutant enzyme Y97M, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.23 | 146.6 | - |
N-acetyl-alpha-D-glucosamine 1-phosphate | wild-type enzyme, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.7.7.23 | 338.4 | - |
N-acetyl-alpha-D-glucosamine 1-phosphate | mutant enzyme Y97N, pH 7.5, 80°C | Sulfurisphaera tokodaii |