EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.2.3 | T185N | NCR-truncated protein, mutant has lost its M-producing capability but retained the ability to yield L-guluronate units by almost completely degrading 2-aminobenzamide-labeled tetra(L-guluronate) chains. Mutant T185N can degrade 2-aminobenzamide-labeled penta(L-guluronate) and 2-aminobenzamide-labeled penta(D-mannuronate) fractions at greater proportions than wild-type | Flammeovirga sp. MY04 |
4.2.2.11 | T185N | NCR-truncated protein, mutant has lost its M-producing capability but retained the ability to yield G units by almost completely degrading 2-aminobenzamide-labeled G4 chains. Mutant T185N can degrade 2-aminobenzamide-labeled G5 and 2-aminobenzamide-labeled M5 fractions at greater proportions than wild-type | Flammeovirga sp. MY04 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.2.3 | Flammeovirga sp. MY04 | A0A1B1FUW4 | bifunctional alginate lyase, catalyzes the reactions of EC 4.2.2.3 and EC 4.2.2.11 | - |
4.2.2.11 | Flammeovirga sp. MY04 | A0A1B1FUW4 | bifunctional alginate lyase, catalyzes the reactions of EC 4.2.2.3 and EC 4.2.2.11 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.3 | alginate | - |
Flammeovirga sp. MY04 | unsaturated algino-oligosaccharides | - |
? | |
4.2.2.3 | additional information | Aly1 is a bifunctional alginate lyase and prefers L-guluronate to D-mannuronate. Tetrasaccharide-size fractions are the smallest substrates, and D-mannuronate, L-guluronate, and UDP2 fractions are the minimal product types. Products are a series of small size-defined saturated oligosaccharide products from the nonreducing ends of single or different saturated sugar chains and yielding unsaturated products in distinct but restricted pattern. No substrates: chondroitin, chondroitin sulfate, dermantan sulfate B, hyaluronan, heparin, or heparin sulfate | Flammeovirga sp. MY04 | ? | - |
? | |
4.2.2.3 | poly(alpha-L-(1,4)-guluronate) | reaction of EC 4.2.2.11 | Flammeovirga sp. MY04 | 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? | |
4.2.2.3 | poly(beta-D-mannuronate) | - |
Flammeovirga sp. MY04 | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) | - |
? | |
4.2.2.11 | alginate | - |
Flammeovirga sp. MY04 | unsaturated algino-oligosaccharides | - |
? | |
4.2.2.11 | additional information | Aly1 is a bifunctional alginate lyase and prefers G to M. Tetrasaccharide-size fractions are the smallest substrates, and D-mannuronate, L-guluronate, and UDP2 fractions are the minimal product types. Products are a series of small size-defined saturated oligosaccharide products from the nonreducing ends of single or different saturated sugar chains and yielding unsaturated products in distinct but restricted pattern. No substrates: chondroitin, chondroitin sulfate, dermantan sulfate B, hyaluronan, heparin, or heparin sulfate | Flammeovirga sp. MY04 | ? | - |
? | |
4.2.2.11 | poly(alpha-L-1,4-guluronate) | - |
Flammeovirga sp. MY04 | 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? | |
4.2.2.11 | poly(beta-D-mannuronate) | - |
Flammeovirga sp. MY04 | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.2.3 | ? | x * 50650, calculated from sequence | Flammeovirga sp. MY04 |
4.2.2.11 | ? | x * 50650, calculated from sequence | Flammeovirga sp. MY04 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.2.3 | Aly1 | - |
Flammeovirga sp. MY04 |
4.2.2.11 | Aly1 | - |
Flammeovirga sp. MY04 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 50 | - |
- |
Flammeovirga sp. MY04 |
4.2.2.11 | 50 | - |
- |
Flammeovirga sp. MY04 |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | - |
40 | 24 h, at least 60% residual activity | Flammeovirga sp. MY04 |
4.2.2.11 | - |
40 | 24 h, at least 60% residual activity | Flammeovirga sp. MY04 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 6 | - |
- |
Flammeovirga sp. MY04 |
4.2.2.11 | 6 | - |
- |
Flammeovirga sp. MY04 |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 5 | 8 | 2 h, at least 80% residual activity | Flammeovirga sp. MY04 |
4.2.2.11 | 5 | 8 | 2 h, at least 80% residual activity | Flammeovirga sp. MY04 |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
4.2.2.3 | Flammeovirga sp. MY04 | calculated from sequence | - |
4.2 |
4.2.2.11 | Flammeovirga sp. MY04 | calculated from sequence | - |
4.2 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.2.3 | physiological function | deletion of the noncatalytic region of Aly1 causes weak changes of biochemical characteristics but increases the degradation proportions of small size-defined saturated D-mannuronate-enriched oligosaccharide substrates and unsaturated tetrasaccharide fractions without any size changes of degradable oligosaccharides | Flammeovirga sp. MY04 |
4.2.2.11 | physiological function | deletion of the noncatalytic region of Aly1 causes weak changes of biochemical characteristics but increases the degradation proportions of small size-defined saturated M-enriched oligosaccharide substrates and unsaturated tetrasaccharide fractions without any size changes of degradable oligosaccharides | Flammeovirga sp. MY04 |