Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Empadinhas, N.; Albuquerque, L.; Henne, A.; Santos, H.; Da Costa, M.
    The bacterium Thermus thermophilus, like hyperthermophilic archaea, uses a two-step pathway for the synthesis of mannosylglycerate (2003), Appl. Environ. Microbiol., 69, 3272-3279 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.217 overexpressed in Escherichia coli Thermus thermophilus
3.1.3.70 overexpressed in Escherichia coli Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.217 KCl 50 to 300 mM Thermus thermophilus
2.4.1.217 Mn2+ 20 mM Thermus thermophilus
2.4.1.217 NaCl 50 to 300 mM Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.217 0.13
-
 3-phospho-D-glycerate pH 7.6, 70°C Thermus thermophilus
2.4.1.217 0.33
-
 GDP-alpha-D-mannose pH 7.6, 70°C Thermus thermophilus
3.1.3.70 0.52
-
 2-O-(alpha-D-mannosyl)-3-phosphoglycerate pH 7.6, 70°C Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.217 Mg2+ activity is strictly dependent on divalent cations, Mn2+ is most effective (between 0.2 and 2.0 mM), Mg2+x01has a similar effect on the activation at about 20.0 mM Thermus thermophilus
2.4.1.217 Mn2+ activity is strictly dependent on divalent cations, Mn2+ is most effective, maximum activation of the enzyme by Mn2+ is between 0.2 and 2.0 mM Thermus thermophilus
2.4.1.217 additional information Ca2+, Sr2+, Cu2+, Ba2+, and Zn2+, do not stimulate activity at any concentration Thermus thermophilus
3.1.3.70 Co2+ activity is strictly dependent on divalent cations. Mn2+, Co2+, Mg2+, and to a lesser extent Ni2+ activate the enzyme Thermus thermophilus
3.1.3.70 Mg2+ activity is strictly dependent on divalent cations. Mn2+, Co2+, Mg2+, and to a lesser extent Ni2+ activate the enzyme Thermus thermophilus
3.1.3.70 Mn2+ activity is strictly dependent on divalent cations. Mn2+, Co2+, Mg2+, and to a lesser extent Ni2+ activate the enzyme Thermus thermophilus
3.1.3.70 additional information Ca2+, Sr2+, Ba2+, Cu2+, and Zn2+, have no effect on enzyme activity at any concentration Thermus thermophilus
3.1.3.70 Ni2+ activity is strictly dependent on divalent cations. Mn2+, Co2+, Mg2+, and to a lesser extent Ni2+ activate the enzyme Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.217 43600
-
 calculated from sequence Thermus thermophilus
3.1.3.70 28100
-
 calculated from sequence Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.217 Thermus thermophilus Q84B24
-
-
2.4.1.217 Thermus thermophilus DSM 7039 Q84B24
-
-
3.1.3.70 Thermus thermophilus Q84B23
-
-
3.1.3.70 Thermus thermophilus DSM 7039 Q84B23
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.217 GDP-alpha-D-mannose + 3-phospho-D-glycerate
-
 Thermus thermophilus GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
 ?
2.4.1.217 GDP-alpha-D-mannose + 3-phospho-D-glycerate
-
 Thermus thermophilus DSM 7039 GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
 ?
3.1.3.70 2-O-(alpha-D-mannosyl)-3-phosphoglycerate + H2O high substrate specificity, 2-O-(alpha-D-mannosyl)-3-phosphoglycerate is the only substrate dephosphorylated by the enzyme Thermus thermophilus 2-O-(alpha-D-mannosyl)-D-glycerate + phosphate
-
 ?
3.1.3.70 2-O-(alpha-D-mannosyl)-3-phosphoglycerate + H2O high substrate specificity, 2-O-(alpha-D-mannosyl)-3-phosphoglycerate is the only substrate dephosphorylated by the enzyme Thermus thermophilus DSM 7039 2-O-(alpha-D-mannosyl)-D-glycerate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.217 MPGS
-
 Thermus thermophilus
3.1.3.70 MPGP
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.217 80 90
-
 Thermus thermophilus
3.1.3.70 90 95
-
 Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.217 25 103 25°C: 10% of the maximal activity, 103°C: 55% of maximal activity Thermus thermophilus
3.1.3.70 30 103 30°C: about 15% of maximal activity, 103°C: about 70% of maximal activity Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.217 80
-
 half-life: 189 min Thermus thermophilus
2.4.1.217 90
-
 half-life: 22 min Thermus thermophilus
2.4.1.217 100
-
 half-life: 10 min Thermus thermophilus
3.1.3.70 90
-
 half-life: 25 min Thermus thermophilus
3.1.3.70 100
-
 half-life: 2 min Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.217 7
-
-
 Thermus thermophilus
3.1.3.70 6
-
-
 Thermus thermophilus