Any feedback?
Literature summary extracted from
- Bioproduction of L-aspartic acid and cinnamic Acid by L-aspartate ammonia lyase from Pseudomonas aeruginosa PAO1 (2017), Appl. Biochem. Biotechnol., 182, 792-803 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.3.1.1 | synthesis | bioproduction of L-aspartic acid and cinnamic acid. From an initial concentration of 1000 mM of fumarate and 30 mM of L-phenylalanine, the enzyme converts 0.395 mM L-aspartic acid and 3.47 mM cinnamic acid, respectively | Pseudomonas aeruginosa |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.3.1.1 | expression in Escherichia coli BL21 | Pseudomonas aeruginosa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.3.1.1 | Hg2+ | - |
Pseudomonas aeruginosa |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.1.1 | 0.346 | - |
L-aspartate | pH 8.0, 35°C | Pseudomonas aeruginosa | |
| 4.3.1.1 | 1.71 | - |
L-phenylalanine | pH 8.0, 35°C | Pseudomonas aeruginosa | |
| 4.3.1.1 | 2000 | - |
fumarate | pH 8.0, 35°C | Pseudomonas aeruginosa | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.3.1.1 | Mg2+ | the enzyme does not require metal ion for its activity. It is activated in the presence of metal ions. Mg2+ is the most effective | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.1 | 51000 | - |
recombinant His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.1.1 | Pseudomonas aeruginosa | Q9HTD7 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.3.1.1 | - |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.1 | 5.42 | - |
substrate: fumarate, pH 8.0, 35°C | Pseudomonas aeruginosa |
| 4.3.1.1 | 31.21 | - |
substrate: L-aspartate, pH 8.0, 35°C | Pseudomonas aeruginosa |
| 4.3.1.1 | 38.35 | - |
substrate: L-phenylalanine, pH 8.0, 35°C | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.1.1 | fumarate + NH3 | - |
Pseudomonas aeruginosa | L-aspartate | - |
r | |
| 4.3.1.1 | L-aspartate | - |
Pseudomonas aeruginosa | fumarate + NH3 | - |
r | |
| 4.3.1.1 | L-phenylalanine | - |
Pseudomonas aeruginosa | cinnamic acid + NH3 | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.3.1.1 | ? | x * 51000, recombinant His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.3.1.1 | L-aspartate ammonia lyase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.1 | 35 | - |
deamination reaction | Pseudomonas aeruginosa |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.1 | 15 | 40 | 15°C: about 80% of maximal activity, 30°C: about 40% of maximal activity, 35: maximal activity, 40°C: about 90% of maximal activity, 50°C: about 30% of maximal activity | Pseudomonas aeruginosa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.1 | 35 | - |
the enzyme retains 56% activity after 7 days of incubation | Pseudomonas aeruginosa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.1.1 | 4.908 | - |
L-aspartate | pH 8.0, 35°C | Pseudomonas aeruginosa | |
| 4.3.1.1 | 8.002 | - |
L-phenylalanine | pH 8., 35°C | Pseudomonas aeruginosa | |
| 4.3.1.1 | 13.64 | - |
fumarate | pH 8.0, 35°C | Pseudomonas aeruginosa | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.1 | 8 | - |
deamination reaction | Pseudomonas aeruginosa |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.1 | 5 | 8 | pH 5.0: about 80% of maximal activity, pH 8.0: maximal activity, pH 9.0: about 25% of maximal activity, deamination reaction | Pseudomonas aeruginosa |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.1.1 | 0.038 | - |
fumarate | pH 8.0, 35°C | Pseudomonas aeruginosa | |
| 4.3.1.1 | 4.65 | - |
L-phenylalanine | pH 8.0, 35°C | Pseudomonas aeruginosa | |
| 4.3.1.1 | 14.18 | - |
L-aspartate | pH 8.0, 35°C | Pseudomonas aeruginosa | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
