Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhu, C.; Zhang, J.; Li, L.; Zhang, J.; Jiang, Y.; Shen, Z.; Guan, H.; Jiang, X.
    Purification and characterization of hyaluronate lyase from Arthrobacter globiformis A152 (2017), Appl. Biochem. Biotechnol., 182, 216-228 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.1 1,10-phenanthroline 5 mM, 75% residual activity Arthrobacter globiformis
4.2.2.1 Cu2+ 50 mM, no residual activity Arthrobacter globiformis
4.2.2.1 EDTA 5 mM, 84% residual activity Arthrobacter globiformis
4.2.2.1 hyaluronate substrate inhibition above 7.5 mg/ml Arthrobacter globiformis
4.2.2.1 Li+ 50 mM, 68% residual activity Arthrobacter globiformis
4.2.2.1 Mg2+ 50 mM, 68% residual activity Arthrobacter globiformis
4.2.2.1 SDS 5 mM, 4.6% residual activity Arthrobacter globiformis
4.2.2.1 Tween 80 5 mM, 84% residual activity Arthrobacter globiformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.1 additional information
-
 hyaluronate Km value 0.11 mg/ml, pH 6.5, 37°C Arthrobacter globiformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.1 Ca2+ 50 mM, 111% of initial activity Arthrobacter globiformis

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.1 Arthrobacter globiformis
-
-
-
4.2.2.1 Arthrobacter globiformis A152
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.1 purification from the supernatant by ammonium sulfate fractionation, Q Sepharose Fast Flow, and Sephadex G-100 chromatography Arthrobacter globiformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.1 297.2
-
 pH 6.5, 37°C Arthrobacter globiformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.1 chondroitin sulfate A 24.7% of the activity with hyaluronate Arthrobacter globiformis ?
-
 ?
4.2.2.1 chondroitin sulfate A 24.7% of the activity with hyaluronate Arthrobacter globiformis A152 ?
-
 ?
4.2.2.1 hyaluronate
-
 Arthrobacter globiformis 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
-
 ?
4.2.2.1 hyaluronate
-
 Arthrobacter globiformis A152 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
-
 ?
4.2.2.1 additional information very poor substrate: chitosan. No substrates: heparin sodium, sodium alginate, carrageenan, pectin, soluble starch, xanthan gum Arthrobacter globiformis ?
-
 ?
4.2.2.1 additional information very poor substrate: chitosan. No substrates: heparin sodium, sodium alginate, carrageenan, pectin, soluble starch, xanthan gum Arthrobacter globiformis A152 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.1 ? x * 73700, SDS-PAGE Arthrobacter globiformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.1 42
-
-
 Arthrobacter globiformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.1 25 50 at least 47% of maximum activity Arthrobacter globiformis
4.2.2.1 37
-
 more than 76% of maximum activity Arthrobacter globiformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.1 30 37 120 min, more than 84% residual activity Arthrobacter globiformis
4.2.2.1 42
-
 60 min, 43% residual activity Arthrobacter globiformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.1 6
-
-
 Arthrobacter globiformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.1 5 9
-
 Arthrobacter globiformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.1 5 10 1 h, 37°C, 81% residual activity Arthrobacter globiformis