EC Number | Application | Comment | Organism |
---|---|---|---|
4.2.1.92 | synthesis | the recombinant 13-HPL enzyme is valuated as biocatalyst to produce C6-aldehydes | Olea europaea |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.92 | a cDNA encoding for olive HPL of Leccino variety is isolated from black olive fruits, cloned in pQE-30 expression vector, and expressed as His-tagged proteins in Escherichia coli strain M15. In order to improve the enzyme solubility, the chloroplast transit peptide is deleted | Olea europaea |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.92 | additional information | the recombinant 13-HPL enzyme is assayed as biocatalyst to produce C6-aldehydes, optimization of the biotransformation conditions of (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid and (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid into hexanal and (Z)-3-hexenal, respectively, to achieve the highest yields. The wild-type full-length enzyme acts more effectively on (9Z,11E,15Z)-(13S)-hydroperoxyoctadeca-9,11,15-trienoate and (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid than on the truncated enzyme lacking the chloroplast transit peptide | Olea europaea |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.92 | 0.0431 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.5, 25°C, recombinant full-length enzyme | Olea europaea | |
4.2.1.92 | 0.04322 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.5, 25°C, recombinant truncated enzyme | Olea europaea | |
4.2.1.92 | 0.1528 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.5, 25°C, recombinant full-length enzyme | Olea europaea | |
4.2.1.92 | 0.1739 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.5, 25°C, recombinant truncated enzyme | Olea europaea |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.1.92 | chloroplast envelope | the enzyme contains a chloroplast transit peptide in its N-terminal end for targeting and insertion to the chloroplast envelope | Olea europaea | 9941 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.92 | (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | Olea europaea | - |
hexanal + 12-oxo-9-dodecenoic acid | - |
? | |
4.2.1.92 | (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | Olea europaea | - |
(Z)-3-hexenal + 12-oxo-9-dodecenoic acid | - |
? | |
4.2.1.92 | additional information | Olea europaea | according to their specificity, HPLs can act on the 9- or 13-hydroperoxides of linoleic and linolenic acids (hydroperoxyoctadecadienoic acid (HPOD) and hydroperoxyoctadecatrienoic acid (HPOT)) and catalyze the cleavage of these hydroperoxides into C6- or C9-aldehydes and the C12- or C9-oxo-acids, respectively | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.92 | Olea europaea | G8XQN2 | Leccino variety | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.92 | recombinant His-tagged full-length and truncated enzymes from Escherichia coli strain M15 by cobalt affinity chromatography | Olea europaea |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.1.92 | fruit | black olives of the Leccino variety harvested at 20 weeks after flowering. Olive HPL is active in a range of pH between 6.0 and 8.0 with an optimal pH at 6.5 and an optimal production of C6 compounds performed at 20°C | Olea europaea | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.92 | (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | - |
Olea europaea | hexanal + 12-oxo-9-dodecenoic acid | - |
? | |
4.2.1.92 | (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | - |
Olea europaea | (Z)-3-hexenal + 12-oxo-9-dodecenoic acid | - |
? | |
4.2.1.92 | additional information | according to their specificity, HPLs can act on the 9- or 13-hydroperoxides of linoleic and linolenic acids (hydroperoxyoctadecadienoic acid (HPOD) and hydroperoxyoctadecatrienoic acid (HPOT)) and catalyze the cleavage of these hydroperoxides into C6- or C9-aldehydes and the C12- or C9-oxo-acids, respectively | Olea europaea | ? | - |
? | |
4.2.1.92 | additional information | the recombinant 13-HPL enzyme is assayed as biocatalyst to produce C6-aldehydes. Bioconversion of 13-hydroperoxides of linoleic and linolenic acids by recombinant enzyme with or without chloroplast targeting signal sequence, reaction products are identified and quantified using gas chromatography and mass spectrometry. Recombinant HPL enzyme with targeting sequence allows production of 5.61 mM hexanal and 4.39 mM (Z)-3-hexenal, corresponding to high conversion yields of 93.5% and 73%, respectively. The HPL enzyme without targeting sequence fails to obtain greater quantities of hexanal or (Z)-3-hexenal. No undesirable products are formed, and no isomerization of (Z)-3-hexenal in (E)-2-hexenal occurs | Olea europaea | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.92 | ? | x * 50000, about, recombinant enzyme with or without transit peptide, SDS-PAGE | Olea europaea |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.92 | 13-HPL | - |
Olea europaea |
4.2.1.92 | 13-hydroperoxide lyase | - |
Olea europaea |
4.2.1.92 | HPL | - |
Olea europaea |
4.2.1.92 | hydroperoxide lyase | - |
Olea europaea |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.92 | 25 | - |
recombinant enzyme | Olea europaea |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.92 | 17.89 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.5, 25°C, recombinant truncated enzyme | Olea europaea | |
4.2.1.92 | 26.38 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.5, 25°C, recombinant full-length enzyme | Olea europaea | |
4.2.1.92 | 402.62 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.5, 25°C, recombinant truncated enzyme | Olea europaea | |
4.2.1.92 | 564.95 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.5, 25°C, recombinant full-length enzyme | Olea europaea |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.92 | 7.5 | - |
recombinant enzyme | Olea europaea |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.92 | 6.5 | 8 | purified recombinant enzyme, over 80% activity remaining | Olea europaea |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.1.92 | evolution | hydroperoxide lyases are classified in the cytochrome P450 family and more particularly in the two subfamilies CYP74B (13-HPL) and CYP74C (9-HPL and 9/13-HPL) | Olea europaea |
4.2.1.92 | metabolism | the enzyme is involved in the lipoxygenase (LOX) pathway | Olea europaea |
4.2.1.92 | physiological function | green leaf volatiles (GLVs) are the main contributors to the characteristic odor of plants known under the name of green note. GLVs are naturally produced through a metabolic pathway of the C18-polyunsaturated fatty acids named the lipoxygenase pathway. First, the lipoxygenase (LOX) catalyzes the oxygenation of linoleic and linolenic acids to form corresponding fatty acid hydroperoxides which are then cleaved by hydroperoxide lyase (HPL) to generate short-chain aldehydes and oxoacids. GLVs are widely used by various industries to improve food quality by restoring the fresh and fruity green odor or to add a touch of freshness to fragrances. The C6-aldehyde compounds are the main components (about 80 %) of the virgine olive oil flavor | Olea europaea |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.92 | 413.9 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.5, 25°C, recombinant truncated enzyme | Olea europaea | |
4.2.1.92 | 612.1 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.5, 25°C, recombinant full-length enzyme | Olea europaea | |
4.2.1.92 | 2315.2 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.5, 25°C, recombinant truncated enzyme | Olea europaea | |
4.2.1.92 | 3697.3 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.5, 25°C, recombinant full-length enzyme | Olea europaea |