Any feedback?
Literature summary extracted from
- Heterologous expression, purification, and biochemical characterization of alpha-humulene synthase from Zingiber zerumbet Smith (2016), Appl. Biochem. Biotechnol., 178, 474-489 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.3.104 | gene ZSS1 or hum, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) under control of the lac promoter | Zingiber zerumbet |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.3.104 | additional information | alpha-humulene production using the engineered Escherichia coli BL21(DE3) strain in a 2-l bioreactor cultivation, method optimization, overview. Coordinating Cu2+ ions to the NTA ligands in affinity chromatography results in a high non-specific binding of endogenous host cell proteins due to the high affinity of Cu2+ to histidine groups. Zn2+-IMAC shows much less impurities in eluted protein fractions which is consistent with the fact that Zn2+ ions are less affine to histidine groups. The recovery rate of 80 % of the protein of interest using Zn2+ ions is the lowest compared to all other metal ions tested. Ni2+- and Co2+-IMAC seem to be similar in product purity (60% and 63%) and recovery (93% and 95%). As Co2+-IMAC shows the best purity to recovery ratio, further purification is done using Co2+-IMAC | Zingiber zerumbet |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.3.104 | additional information | - |
additional information | Michaelis-Menten kinetics, overview | Zingiber zerumbet | |
| 4.2.3.104 | 0.033 | - |
(2E,6E)-farnesyl diphosphate | pH 7.0, 38°C, recombinant enzyme | Zingiber zerumbet |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.104 | (2E,6E)-farnesyl diphosphate | Zingiber zerumbet | - |
alpha-humulene + diphosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.3.104 | Zingiber zerumbet | B1B1U3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.3.104 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)by cobalt affinity chromatography, dialysis, and ultrafiltration, to homogeneity | Zingiber zerumbet |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.104 | (2E,6E)-farnesyl diphosphate | - |
Zingiber zerumbet | alpha-humulene + diphosphate | - |
? | |
| 4.2.3.104 | additional information | the recombinant alpha-humulene synthase produces 93.45% alpha-humulene and 5.5% beta-caryophyllene. Product determination by gas chromatography-flame ionization detection (GC-FID) | Zingiber zerumbet | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.3.104 | ? | x * 64500, about, sequence calculation | Zingiber zerumbet |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.3.104 | hum | - |
Zingiber zerumbet |
| 4.2.3.104 | ZSS1 | - |
Zingiber zerumbet |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.104 | 38 | - |
recombinant His-tagged enzyme | Zingiber zerumbet |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.104 | 32 | 44 | activity range, profile overview, recombinant His-tagged enzyme | Zingiber zerumbet |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.3.104 | 3.8 | - |
(2E,6E)-farnesyl diphosphate | pH 7.0, 38°C, recombinant enzyme | Zingiber zerumbet | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.104 | 7.5 | - |
recombinant His-tagged enzyme | Zingiber zerumbet |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.104 | 6 | 9 | activity range, profile overview, recombinant His-tagged enzyme | Zingiber zerumbet |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 4.2.3.104 | Zingiber zerumbet | recombinant His-tagged enzyme, isoelectric focusing | - |
5.61 |
| 4.2.3.104 | Zingiber zerumbet | sequence calculation | - |
5.65 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.2.3.104 | metabolism | the enzyme is involved in the zerumbone biosynthesis in Zingiber zerumbet, proposed pathway and mechanism, overview | Zingiber zerumbet |
| 4.2.3.104 | physiological function | alpha-humulene is a common sesquiterpene widely distributed in plants, the synthase from Zingiber zerumbet is the only known sesquiterpene synthase to date catalyzing the formation of alpha-humulene as major product | Zingiber zerumbet |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
