Literature summary extracted from

Huerlimann, L.M.; Corradi, V.; Hohl, M.; Bloemberg, G.V.; Tieleman, D.P.; Seeger, M.A.
The heterodimeric ABC transporter EfrCD mediates multidrug efflux in Enterococcus faecalis (2016), Antimicrob. Agents Chemother., 60, 5400-5411 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
7.6.2.2	expressed in Lactococcus lactis strain NZ9000	Enterococcus faecalis

Protein Variants

EC Number	Protein Variants	Comment	Organism
7.6.2.2	E512Q	inactive	Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
7.6.2.2	ATP + H2O + xenobiotic[side 1]	Enterococcus faecalis	-	ADP + phosphate + xenobiotic[side 2]	-	?
7.6.2.2	ATP + H2O + xenobiotic[side 1]	Enterococcus faecalis 4205	-	ADP + phosphate + xenobiotic[side 2]	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
7.6.2.2	Enterococcus faecalis	-	-	-
7.6.2.2	Enterococcus faecalis 4205	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
7.6.2.2	Ni-NTA column chromatography and Superdex 200 gel filtration	Enterococcus faecalis

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7.6.2.2	ATP + H2O + acriflavine [side 1]	-	Enterococcus faecalis	ADP + phosphate + acriflavine[side 2]	-	?
7.6.2.2	ATP + H2O + acriflavine [side 1]	-	Enterococcus faecalis 4205	ADP + phosphate + acriflavine[side 2]	-	?
7.6.2.2	ATP + H2O + ciprofloxacin[side 1]	-	Enterococcus faecalis	ADP + phosphate + ciprofloxacin[side 2]	-	?
7.6.2.2	ATP + H2O + ciprofloxacin[side 1]	-	Enterococcus faecalis 4205	ADP + phosphate + ciprofloxacin[side 2]	-	?
7.6.2.2	ATP + H2O + daunorubicin[side 1]	-	Enterococcus faecalis	ADP + phosphate + daunorubicin[side 2]	-	?
7.6.2.2	ATP + H2O + daunorubicin[side 1]	-	Enterococcus faecalis 4205	ADP + phosphate + daunorubicin[side 2]	-	?
7.6.2.2	ATP + H2O + doxorubicin[side 1]	-	Enterococcus faecalis	ADP + phosphate + doxorubicin[side 2]	-	?
7.6.2.2	ATP + H2O + doxorubicin[side 1]	-	Enterococcus faecalis 4205	ADP + phosphate + doxorubicin[side 2]	-	?
7.6.2.2	ATP + H2O + ethidium[side 1]	-	Enterococcus faecalis	ADP + phosphate + ethidium[side 2]	-	?
7.6.2.2	ATP + H2O + gentamicin[side 1]	-	Enterococcus faecalis	ADP + phosphate + gentamicin[side 2]	-	?
7.6.2.2	ATP + H2O + Hoechst 33342[side 1]	-	Enterococcus faecalis	ADP + phosphate + Hoechst 33342[side 2]	-	?
7.6.2.2	ATP + H2O + kanamycin[side 1]	-	Enterococcus faecalis	ADP + phosphate + kanamycin[side 2]	-	?
7.6.2.2	ATP + H2O + minocycline[side 1]	-	Enterococcus faecalis	ADP + phosphate + minocycline[side 2]	-	?
7.6.2.2	ATP + H2O + norfloxacin[side 1]	-	Enterococcus faecalis	ADP + phosphate + norfloxacin[side 2]	-	?
7.6.2.2	ATP + H2O + ofloxacin[side 1]	-	Enterococcus faecalis	ADP + phosphate + ofloxacin[side 2]	-	?
7.6.2.2	ATP + H2O + rifampin[side 1]	-	Enterococcus faecalis	ADP + phosphate + rifampin[side 2]	-	?
7.6.2.2	ATP + H2O + tetracycline[side 1]	-	Enterococcus faecalis	ADP + phosphate + tetracycline[side 2]	-	?
7.6.2.2	ATP + H2O + xenobiotic[side 1]	-	Enterococcus faecalis	ADP + phosphate + xenobiotic[side 2]	-	?
7.6.2.2	ATP + H2O + xenobiotic[side 1]	-	Enterococcus faecalis 4205	ADP + phosphate + xenobiotic[side 2]	-	?

Subunits

EC Number	Subunits	Comment	Organism
7.6.2.2	heterodimer	-	Enterococcus faecalis

Synonyms

EC Number	Synonyms	Comment	Organism
7.6.2.2	EfrAB	-	Enterococcus faecalis
7.6.2.2	EfrCD	-	Enterococcus faecalis
7.6.2.2	EfrEE	-	Enterococcus faecalis
7.6.2.2	multidrug ABC transporter	-	Enterococcus faecalis
7.6.2.2	multidrug transporter	-	Enterococcus faecalis

General Information

EC Number	General Information	Comment	Organism
7.6.2.2	malfunction	enzyme deletion results in increased susceptibility to daunorubicin, doxorubicin, ethidium, and Hoechst 33342	Enterococcus faecalis

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Release 2023.1 (January 2023)