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Literature summary extracted from
- Direct participation of a peripheral side chain of a corrin ring in coenzyme B12 catalysis (2018), Angew. Chem. Int. Ed. Engl., 57, 7830-7835 .
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.2.1.28 | structures in the absence and presence of substrates at resolutions of 1.55-2.05 A | Klebsiella oxytoca |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.1.28 | S224A | mutation in alpha-subunit, a key residue for stabilizing the post-homolysis state of the adenosyl group. More than 5fold reduction in kcat/Km | Klebsiella oxytoca |
| 4.2.1.28 | S224C | mutation in alpha-subunit, a key residue for stabilizing the post-homolysis state of the adenosyl group. About 100fold reduction in kcat/Km | Klebsiella oxytoca |
| 4.2.1.28 | S224N | mutation in alpha-subunit, a key residue for stabilizing the post-homolysis state of the adenosyl group. More than 100fold reduction in kcat/Km | Klebsiella oxytoca |
| 4.2.1.28 | T172A | mutation in alpha-subunit, a key residue for stabilizing the post-homolysis state of the adenosyl group. More than 100fold reduction in kcat/Km | Klebsiella oxytoca |
| 4.2.1.28 | T172S | mutation in alpha-subunit, a key residue for stabilizing the post-homolysis state of the adenosyl group. More than 10fold reduction in kcat/Km | Klebsiella oxytoca |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.28 | 0.031 | - |
Propane-1,2-diol | mutant T172A, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca |  |
| 4.2.1.28 | 0.15 | - |
Propane-1,2-diol | wild-type, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
| 4.2.1.28 | 0.15 | - |
Propane-1,2-diol | mutant S224A, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
| 4.2.1.28 | 0.16 | - |
Propane-1,2-diol | mutant T172S, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
| 4.2.1.28 | 0.51 | - |
Propane-1,2-diol | mutant S224C, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
| 4.2.1.28 | 1.9 | - |
Propane-1,2-diol | mutant S224N, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.28 | Klebsiella oxytoca | Q59470 | alpha subunit | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.28 | propane-1,2-diol | - |
Klebsiella oxytoca | propanal + H2O | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.28 | 0.5 | - |
Propane-1,2-diol | mutant T172A, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
| 4.2.1.28 | 12 | - |
Propane-1,2-diol | mutant S224C, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
| 4.2.1.28 | 17 | - |
Propane-1,2-diol | mutant S224N, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
| 4.2.1.28 | 34 | - |
Propane-1,2-diol | mutant T172S, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
| 4.2.1.28 | 64 | - |
Propane-1,2-diol | mutant S224A, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
| 4.2.1.28 | 336 | - |
Propane-1,2-diol | wild-type, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.28 | adenosylcobalamin | Km value 0.15 mM for wild-type | Klebsiella oxytoca | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.28 | 9 | - |
Propane-1,2-diol | mutant S224N, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
| 4.2.1.28 | 16 | - |
Propane-1,2-diol | mutant T172A, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
| 4.2.1.28 | 24 | - |
Propane-1,2-diol | mutant S224C, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
| 4.2.1.28 | 210 | - |
Propane-1,2-diol | mutant T172S, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
| 4.2.1.28 | 430 | - |
Propane-1,2-diol | mutant S224A, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
| 4.2.1.28 | 2200 | - |
Propane-1,2-diol | wild-type, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
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