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Literature summary extracted from

  • Krieg, T.; Sydow, A.; Faust, S.; Huth, I.; Holtmann, D.
    CO2 to terpenes autotrophic and electroautotrophic alpha-humulene production with Cupriavidus necator (2018), Angew. Chem. Int. Ed. Engl., 57, 1879-1882 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.3.104 synthesis chemolithoautotrophic production of a terpene from carbon dioxide, hydrogen, and oxygen, involving the engineered Cupriavidus necator cells and enzyme alpha-humulene synthase, is a promising starting point for the production of different high-value terpene compounds from abundant and simple raw materials. The production system is used to produce 17 mg alpha-humulene per gram cell dry mass (CDW) from CO2 and electrical energy in microbial electrosynthesis (MES) mode Cupriavidus necator

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.104 additional information heterologous expression of the mevalonate pathway and alpha-humulene synthase results in the production of alpha-humulene from CO2 under heterotrophic conditions. The production system is used to produce 17 mg alpha-humulene per gram cell dry mass (CDW) from CO2 and electrical energy in microbial electrosynthesis (MES) mode Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.104 (2E,6E)-farnesyl diphosphate Cupriavidus necator
-
 alpha-humulene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.104 Cupriavidus necator
-
 i.e. Ralstonia eutropha or Alcaligenes eutrophus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.104 (2E,6E)-farnesyl diphosphate
-
 Cupriavidus necator alpha-humulene + diphosphate
-
 ?