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Literature summary extracted from

  • Deng, H.; Botting, C.; Hamilton, J.; Russell, R.; OHagan, D.
    S-adenosyl-L-methionine Hydroxide adenosyltransferase A SAM enzyme (2008), Angew. Chem. Int. Ed. Engl., 47, 5357-5361 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.13.2.3 expressed in Escherichia coli Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.2.3 0.0392
-
 S-adenosyl-L-methionine at pH 8.5 and 37°C Pyrococcus horikoshii
3.13.2.3 0.41
-
 5'-chloro-5'-deoxyadenosine at pH 8.5 and 37°C Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.2.3 S-adenosyl-L-methionine + H2O Pyrococcus horikoshii
-
 adenosine + L-methionine + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.13.2.3 Pyrococcus horikoshii O58212
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.2.3 5'-chloro-5'-deoxyadenosine + H2O low activity Pyrococcus horikoshii ?
-
 ?
3.13.2.3 additional information no activity with 5'-fluoro-5'-deoxyadenosine Pyrococcus horikoshii ?
-
 ?
3.13.2.3 S-adenosyl-L-methionine + H2O
-
 Pyrococcus horikoshii adenosine + L-methionine + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.13.2.3 duf62
-
 Pyrococcus horikoshii
3.13.2.3 SAM hydroxide adenosyltransferase
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.13.2.3 37
-
-
 Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.13.2.3 80
-
 the enzyme retains full activity after being heated at 80°C for 30 min and then cooled to 37°C Pyrococcus horikoshii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.13.2.3 0.0052
-
 5'-chloro-5'-deoxyadenosine at pH 8.5 and 37°C Pyrococcus horikoshii
3.13.2.3 0.14
-
 S-adenosyl-L-methionine at pH 8.5 and 37°C Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.13.2.3 8.5
-
-
 Pyrococcus horikoshii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.13.2.3 5 10 the enzyme is inactive below pH 5.0 and above pH 10.0 Pyrococcus horikoshii