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Literature summary extracted from

  • Zhang, X.; Rao, Z.; Li, J.; Zhou, J.; Yang, T.; Xu, M.; Bao, T.; Zhao, X.
    Improving the acidic stability of Staphylococcus aureus alpha-acetolactate decarboxylase in Bacillus subtilis by changing basic residues to acidic residues (2015), Amino Acids, 47, 707-717 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.5 expressed in Bacillus subtilis strain WB600 Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.5 K52D the mutant has almost no variation on enzyme activity, but exhibits improved stability over pH 4.0-5.0 compared to the wild type enzyme Staphylococcus aureus
4.1.1.5 K52E the mutant has almost no variation on enzyme activity, but exhibits improved stability over pH 4.0-5.0 compared to the wild type enzyme Staphylococcus aureus
4.1.1.5 N43D the mutant has almost no variation on enzyme activity, but exhibits improved stability over pH 4.0-5.0 compared to the wild type enzyme Staphylococcus aureus
4.1.1.5 N43D/K52D the mutant shows dramatically improved acidic stability with prolonged half-life of 3.5 h (compared to the wild type of 1.5 h) at pH 4.0 Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.5 0.0177
-
 (2S)-2-hydroxy-2-methyl-3-oxobutanoate at pH 6.0 and 45°C Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.5 (2S)-2-hydroxy-2-methyl-3-oxobutanoate Staphylococcus aureus
-
 (3R)-3-hydroxybutan-2-one + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.5 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.5 Ni-NTA column chromatography Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.5 (2S)-2-hydroxy-2-methyl-3-oxobutanoate
-
 Staphylococcus aureus (3R)-3-hydroxybutan-2-one + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.5 ? x * 28000, SDS-PAGE Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.5 ALDC
-
 Staphylococcus aureus
4.1.1.5 alpha-Acetolactate decarboxylase
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.5 45
-
-
 Staphylococcus aureus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.5 30 50 more than 60% activity between 30 and 50°C Staphylococcus aureus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.5 additional information
-
 the enzyme activity is nearly 90% at -20°C and about 80% from 0 to 35°C after 10 h, while rapid inactivation is observed over 45°C Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.5 6
-
-
 Staphylococcus aureus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.5 4 9 the wild type enzyme has a half-life of 1.5 h at pH 4.0. The enzyme retains more than 75 % activity at pH 7.0-9.0 after preincubation for 10 h, while the enzyme activity at pH 6.0 is 41% after 10 h and decreases much more rapidly at pH 5.0 after 2.5 h Staphylococcus aureus