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Literature summary extracted from

  • Peng, F.; Byers, K.; Bradshaw, H.J.
    Less is more Independent loss-of-function ocimene synthase alleles parallel pollination syndrome diversification in monkeyflowers (Mimulus) (2017), Am. J. Bot., 104, 1055-1059 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.106 gene OS, genotyping and genomic analysis, sequence comparisons, quantitative RT-PCR expression analysis, recombinant overexpression in Escherichia coli strain BL21 Erythranthe lewisii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.106 geranyl diphosphate Erythranthe lewisii
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 (E)-beta-ocimene + diphosphate
-
 ?
4.2.3.106 additional information Erythranthe lewisii in vivo emission of E-beta-ocimene from flowers is very low, but southern race Mimulus lewisii flowers emit significantly more E-beta-ocimene than northern race flowers ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.106 Erythranthe lewisii W6A3F2 i.e. Erythranthe lewisii, southern race distributed in the Sierra Nevada
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.106 flower
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 Erythranthe lewisii
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.106 geranyl diphosphate
-
 Erythranthe lewisii (E)-beta-ocimene + diphosphate
-
 ?
4.2.3.106 additional information GC-MS product analysis Erythranthe lewisii ?
-
 ?
4.2.3.106 additional information in vivo emission of E-beta-ocimene from flowers is very low, but southern race Mimulus lewisii flowers emit significantly more E-beta-ocimene than northern race flowers Erythranthe lewisii ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.106 ocimene synthase
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 Erythranthe lewisii

General Information

EC Number General Information Comment Organism
4.2.3.106 evolution the taxa closely related to Mimulus lewisii have evolved several diff erent pollination syndromes, including hummingbird pollination and self pollination (autogamy), floral scent variation contributing to species diversification in this clade, E-beta-ocimene emission within this Mimulus clade does only occur in Mimulus lewisii, given the very low E-beta-ocimene emission in vivo, Mimulus parishii has a fully functional ocimene synthase enzyme in vitro, the other taxa do not emit E-beta- ocimene from flowers, overview Erythranthe lewisii
4.2.3.106 physiological function E-beta-ocimene, a monoterpene produced by ocimene synthase (OS) in Mimulus lewisii, is a floral scent important in attracting the species' bumblebee pollinators Erythranthe lewisii