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Literature summary extracted from

  • Inoue, A.; Satoh, A.; Morishita, M.; Tokunaga, Y.; Miyakawa, T.; Tanokura, M.; Ojima, T.
    Functional heterologous expression and characterization of mannuronan C5-epimerase from the brown alga Saccharina japonica (2016), Algal Res., 16, 282-291 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.1.3.37 additional information no effect by 1 mM EDTA, and 5 mM 2-mercaptethanol Saccharina japonica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.37 gene c5epi, DNA and amino acid sequence determination and analysis revealing eight partial amino acid sequences, SjC5-I to -VIII, highest frequency of clone SjC5-VI is found and elucidated with respect to full-length cDNA and putative gene structure, sequence comparisons, RT-PCR enzyme expression analysis in brown algae, functional heterologous expression of His-tagged MC5E clone SjC5-VI in Spodoptera frugiperda Sf9 cells via baculovirus transfection system, and protein secretion Saccharina japonica

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.37 additional information no effect by 1 mM EDTA, and 5 mM 2-mercaptethanol Saccharina japonica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.37 Ca2+ required, enhances gelation by 1.7fold following rSjC5-VI activity Saccharina japonica
5.1.3.37 additional information no effect by 1 mM of Mg2+, Sr2+, Li+, and Ba2+ Saccharina japonica
5.1.3.37 NaCl activates best at 300 mM Saccharina japonica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.37 [mannuronan]-beta-D-mannuronate Saccharina japonica
-
 [alginate]-alpha-L-guluronate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.37 Saccharina japonica A8CEP1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.37 recombinant secreted His-tagged MC5E clone SjC5-VI from Spodoptera frugiperda Sf9 cells by dialysis and nickel affinity chromatography Saccharina japonica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.3.37 additional information immunohistochemic analysis and PCR enzyme expression analysis in brown algae Saccharina japonica
-
5.1.3.37 sporophyte
-
 Saccharina japonica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.37 additional information Ca2+-induced gel precipitation assay. 1H-NMR spectroscopy of rSjC5-VI-treated polyM reveals alternate epimerization of beta-D-mannuronic acid to alpha-L-guluronic acid for the MC5E activity in eukaryotes. Gelation is enhanced by changes in the M/G ratio. The optimum in vitro polyM concentration is determined at 0.25% Saccharina japonica ?
-
 ?
5.1.3.37 [mannuronan]-beta-D-mannuronate
-
 Saccharina japonica [alginate]-alpha-L-guluronate
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.3.37 c5epi
-
 Saccharina japonica
5.1.3.37 mannuronan C5-epimerase
-
 Saccharina japonica
5.1.3.37 MC5E
-
 Saccharina japonica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.37 35
-
 recombinant enzyme Saccharina japonica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.1.3.37
-
 60 activity range, profile overview Saccharina japonica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.37 7 8.2 recombinant enzyme Saccharina japonica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.3.37 5.5 10 activity range, profile overview Saccharina japonica

General Information

EC Number General Information Comment Organism
5.1.3.37 metabolism biosynthetic pathway of alginate and the alginate structure involving the enzyme, overview Saccharina japonica
5.1.3.37 physiological function in brown algae, the M/G ratio and the composition of blocks consisting of these residues varies based on several factors, including species, portion (blade, stipe, and rhizoid), season, growth conditions, and habitat. Analysis of expression and enzymatic characterization of brown algal MC5E(s) Saccharina japonica