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Literature summary extracted from

  • Hernandez, K.; Gomez, A.; Joglar, J.; Bujons, J.; Parella, T.; Clapes, P.
    2-Keto-3-deoxy-L-rhamnonate aldolase (YfaU) as catalyst in aldol additions of pyruvate to amino aldehyde derivatives (2017), Adv. Synth. Catal., 359, 2090-2100 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.53 expressed in Escherichia coli M-15[pREP-4] cells Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.53 Mg2+ natural metal cofactor Escherichia coli
4.1.2.53 Ni2+ activates better than Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.53 pyruvate + (S)-lactaldehyde Escherichia coli
-
 2-dehydro-3-deoxy-L-rhamnonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.53 Escherichia coli P76469
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.53 HiTrap column chromatography, and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.53 benzyl (1-oxobutan-2-yl)carbamate + 2-oxopropanoate
-
 Escherichia coli (3S)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxyhexanoate + (3R)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxyhexanoate
-
 ?
4.1.2.53 benzyl (1-oxopropan-2-yl)carbamate + 2-oxopropanoate
-
 Escherichia coli (3S)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxypentanoate + (3R)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxypentanoate
-
 ?
4.1.2.53 benzyl (2-oxoethyl)carbamate + 2-oxopropanoate
-
 Escherichia coli (3S)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxybutanoate + (3R)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxybutanoate
-
 ?
4.1.2.53 benzyl (3-methyl-1-oxobutan-2-yl)carbamate + 2-oxopropanoate
-
 Escherichia coli (3S)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxy-5-methylhexanoate + (3R)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxy-5-methylhexanoate
-
 ?
4.1.2.53 benzyl (3-oxopropyl)carbamate + 2-oxopropanoate
-
 Escherichia coli (3R)-5-[[(benzyloxy)carbonyl]amino]-3-hydroxypentanoate + (3S)-5-[[(benzyloxy)carbonyl]amino]-3-hydroxypentanoate
-
 ?
4.1.2.53 benzyl (4-methyl-1-oxopentan-2-yl)carbamate + 2-oxopropanoate
-
 Escherichia coli (3S)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxy-6-methylheptanoate + (3R)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxy-6-methylheptanoate
-
 ?
4.1.2.53 benzyl 2-formylpyrrolidine-1-carboxylate + 2-oxopropanoate
-
 Escherichia coli (4S)-4-[1-[(benzyloxy)carbonyl]pyrrolidin-2-yl]-4-hydroxy-2-oxobutanoate + (4R)-4-[1-[(benzyloxy)carbonyl]pyrrolidin-2-yl]-4-hydroxy-2-oxobutanoate
-
 ?
4.1.2.53 benzyl [(3S)-3-methyl-1-oxopentan-2-yl]carbamate + 2-oxopropanoate
-
 Escherichia coli (3S,5R)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxy-5-methylheptanoate + (3R,5R)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxy-5-methylheptanoate
-
 ?
4.1.2.53 pyruvate + (S)-lactaldehyde
-
 Escherichia coli 2-dehydro-3-deoxy-L-rhamnonate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.53 YfaU
-
 Escherichia coli