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Literature summary extracted from

  • Yildirim, S.; Zezula, J.; Hudlicky, T.; Witholt, B.; Schmid, A.
    Asymmetric dihydroxylation of cinnamonitrile to trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylonitrile using chlorobenzene dioxygenase in Escherichia coli (pTEZ30) (2004), Adv. Synth. Catal., 346, 933-942 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.14.12.26 synthesis asymmetric dihydroxylation of non-natural cinnamonitrile to trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylonitrile by chlorobenzene dioxygenase in recombinant Escherichia coli JM101 (pTEZ30). Recombinant hosts with a strong expression system, such as Escherichia coli JM101 (pTEZ30), can be used to produce dioxygenases efficiently to performcis-dihydroxylations up to technical scales. The cells should be maintained in ametabolically active state duringthe biotransformation in order to increase and maintain the volumetric productivity for long-term reactions Pseudomonas sp. P51

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.26 chlorobenzene dioxygenase genes tcbAa, tcbAb, tcbAc, and tcbAd are expressed in Escherichia coli JM101 (pTEZ30) under the control of the alk regulatory system of Pseudomonas oleovorans GPo1 Pseudomonas sp. P51

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.26 Pseudomonas sp. P51 Q52383 AND Q52384 AND Q52385 AND Q52386 Q52383: large subunit (tcbAa), Q52384: small subunit (tcbAb), Q52385: ferredoxin (tcbAc), Q52386: NADH-ferredoxin reductase (tcbAd)
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.26 cinnamonitrile + NADH + H+ + O2
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 Pseudomonas sp. P51 trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylonitrile + NAD+
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Synonyms

EC Number Synonyms Comment Organism
1.14.12.26 CDO
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 Pseudomonas sp. P51