BRENDA - Enzyme Database

Identification and functional characterization of alpha-enolase from Taenia pisiformis metacestode

Zhang, S.; Guo, A.; Zhu, X.; You, Y.; Hou, J.; Wang, Q.; Luo, X.; Cai, X.; Acta Trop. 144, 31-40 (2015)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
4.2.1.11
medicine
vaccine with the enzyme can produce a 36.4% protection rate in vaccinated rabbits against experimental challenges from Taenia pisiformis eggs
Taenia pisiformis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.6
-
phosphoenolpyruvate
22°C, pH not specified in the publication
Taenia pisiformis
4.2.1.11
0.77
-
2-phospho-D-glycerate
22°C, pH not specified in the publication
Taenia pisiformis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Taenia pisiformis
T1WGD6
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
4.2.1.11
glycoprotein
sequence contains two putative N-linked glycosylation sites at positions 17-NPT-19 and 177-NFT-179
Taenia pisiformis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
metacestode
bladder wall of the metacestode
Taenia pisiformis
-
4.2.1.11
additional information
enzyme is distributed in the periphery of calcareous corpuscles
Taenia pisiformis
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
11.29
-
substrate phosphoenolpyruvate, 22°C, pH not specified in the publication
Taenia pisiformis
4.2.1.11
30.71
-
substrate 2-phospho-D-glycerate, 22°C, pH not specified in the publication
Taenia pisiformis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
746685
Taenia pisiformis
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
phosphoenolpyruvate + H2O
-
746685
Taenia pisiformis
2-phospho-D-glycerate
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 47000, calculated from sequence, x * 66000, SDS-PAGE
Taenia pisiformis
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.11
Taenia pisiformis
calculated from sequence
-
6.8
Application (protein specific)
EC Number
Application
Commentary
Organism
4.2.1.11
medicine
vaccine with the enzyme can produce a 36.4% protection rate in vaccinated rabbits against experimental challenges from Taenia pisiformis eggs
Taenia pisiformis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.6
-
phosphoenolpyruvate
22°C, pH not specified in the publication
Taenia pisiformis
4.2.1.11
0.77
-
2-phospho-D-glycerate
22°C, pH not specified in the publication
Taenia pisiformis
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
4.2.1.11
glycoprotein
sequence contains two putative N-linked glycosylation sites at positions 17-NPT-19 and 177-NFT-179
Taenia pisiformis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
metacestode
bladder wall of the metacestode
Taenia pisiformis
-
4.2.1.11
additional information
enzyme is distributed in the periphery of calcareous corpuscles
Taenia pisiformis
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
11.29
-
substrate phosphoenolpyruvate, 22°C, pH not specified in the publication
Taenia pisiformis
4.2.1.11
30.71
-
substrate 2-phospho-D-glycerate, 22°C, pH not specified in the publication
Taenia pisiformis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
746685
Taenia pisiformis
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
phosphoenolpyruvate + H2O
-
746685
Taenia pisiformis
2-phospho-D-glycerate
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 47000, calculated from sequence, x * 66000, SDS-PAGE
Taenia pisiformis
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.11
Taenia pisiformis
calculated from sequence
-
6.8
General Information
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
enzyme can bind to gen, binding is competitively inhibited by epsilon-aminocaproic acid. Plasminogen bound to Eno can be converted into active plasmin using host-derived activators
Taenia pisiformis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
enzyme can bind to gen, binding is competitively inhibited by epsilon-aminocaproic acid. Plasminogen bound to Eno can be converted into active plasmin using host-derived activators
Taenia pisiformis