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Literature summary extracted from
- Cloning and characterization of squalene synthase and cycloartenol synthase from Siraitia grosvenorii (2017), Acta Pharm. Sin. B, 7, 215-222 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.5.1.21 | full-length cDNAs of SgSQS is cloned by a rapid amplification of cDNA-ends with polymerase chain reaction approach. Transient expression in Nicotiana benthsmiana | Siraitia grosvenorii |
| 5.4.99.8 | full-length cDNAs of SgCAS is cloned by a rapid amplification of cDNA-ends with polymerase chain reaction approach. Transient expression in Nicotiana benthamiana | Siraitia grosvenorii |
| 5.4.99.8 | gene SgCAS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, gene SgCAS belongs to the cycloartenol synthase cluster, quantitative real-time PCR enzyme expression analysis, transcriptome analysis, recombinant expression in Escherichia coli strain BL21(DE3), transient expression of GFP-tagged SgCAS in Nicotiana benthsmiana lower epidermal cells | Siraitia grosvenorii |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.5.1.21 | cytoplasm | combined in silico prediction and subcellular localization, experiments in tobacco indicate that squalene synthase is probably in the cytoplasm or on the cytoskeleton. The squalene synthase protein has two transmembrane regions in the C-terminal | Siraitia grosvenorii | 5737 | - |
| 5.4.99.8 | cytosol | - |
Siraitia grosvenorii | 5829 | - |
| 5.4.99.8 | cytosol | combined in silico prediction and subcellular localization, experiments in tobacco indicate that cycloartenol synthase is likely located in the nucleus or cytosol | Siraitia grosvenorii | 5829 | - |
| 5.4.99.8 | additional information | recombinant GFP-tagged enzyme SgCAS is likely located in the nucleus or cytosol, recombinant GFP-tagged SgCAS in Nicotiana benthamiana is located in the nucleus or cytosol | Siraitia grosvenorii | - |
- |
| 5.4.99.8 | nucleus | - |
Siraitia grosvenorii | 5634 | - |
| 5.4.99.8 | nucleus | combined in silico prediction and subcellular localization, experiments in tobacco indicate that cycloartenol synthase is likely located in the nucleus or cytosol | Siraitia grosvenorii | 5634 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.8 | (3S)-2,3-epoxy-2,3-dihydrosqualene | Siraitia grosvenorii | - |
cycloartenol | - |
r |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.5.1.21 | Siraitia grosvenorii | K7NBW9 | - |
- |
| 5.4.99.8 | Siraitia grosvenorii | K7NBR1 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.5.1.21 | fruit | significantly higher levels in fruits than in other tissues | Siraitia grosvenorii | - |
| 2.5.1.21 | leaf | low activity | Siraitia grosvenorii | - |
| 2.5.1.21 | root | low activity | Siraitia grosvenorii | - |
| 2.5.1.21 | stem | low activity | Siraitia grosvenorii | - |
| 5.4.99.8 | fruit | - |
Siraitia grosvenorii | - |
| 5.4.99.8 | fruit | significantly higher levels in fruits than in other tissues | Siraitia grosvenorii | - |
| 5.4.99.8 | leaf | low activity | Siraitia grosvenorii | - |
| 5.4.99.8 | additional information | enzyme SgCAS has a significantly higher level in fruits than in other tissues | Siraitia grosvenorii | - |
| 5.4.99.8 | root | low activity | Siraitia grosvenorii | - |
| 5.4.99.8 | seedling | - |
Siraitia grosvenorii | - |
| 5.4.99.8 | stem | low activity | Siraitia grosvenorii | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.8 | (3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Siraitia grosvenorii | cycloartenol | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.5.1.21 | ? | x * 47500, calculated from sequence | Siraitia grosvenorii |
| 5.4.99.8 | ? | x * 87500, about, sequence calculation | Siraitia grosvenorii |
| 5.4.99.8 | ? | x * 87500, calculated from sequence | Siraitia grosvenorii |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.5.1.21 | SgSQS | - |
Siraitia grosvenorii |
| 5.4.99.8 | CAS | - |
Siraitia grosvenorii |
| 5.4.99.8 | SgCAS | - |
Siraitia grosvenorii |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 2.5.1.21 | Siraitia grosvenorii | calculated from sequence | - |
7.15 |
| 5.4.99.8 | Siraitia grosvenorii | sequence calculation | - |
6.14 |
| 5.4.99.8 | Siraitia grosvenorii | calculated from sequence | - |
6.14 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.4.99.8 | evolution | determination of evolutionary relationship of squalene synthases (SQSs) or cycloartneol synthases (CASs) from different plants, phylogenetic trees are constructed, overview | Siraitia grosvenorii |
| 5.4.99.8 | metabolism | cycloartenol synthase (CAS) is a key enzyme in triterpenoid and steroid biosynthesis. Both SgSQS and SgCAS have significantly higher levels in fruits than in other tissues, suggesting that steroids and mogrosides are competitors for the same precursors in fruits | Siraitia grosvenorii |
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