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Literature summary extracted from

  • Asojo, O.; Subramanian, S.; Abendroth, J.; Exley, I.; Lorimer, D.; Edwards, T.; Myler, P.
    Crystal structure of chorismate mutase from Burkholderia phymatum (2018), Acta Crystallogr. Sect. F, 74, 187-192 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.5 gene Bphy_7813, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)-R3 Rosetta Paraburkholderia phymatum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.99.5 purified recombinant enzyme, sitting drop vapor diffusion method, mixing of 400 nl of 22.4 mg/ml protein in 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol, and 1 mM TCEP, with 400 nl reservoir solution containing 20% w/v PEG 3350, 200 mM ammonium formate, pH 6.6, and equilibration against 0.08 ml of reservoir solution, X-ray diffraction structure determination and analysis at 1.95 A resolution Paraburkholderia phymatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.5 Chorismate Paraburkholderia phymatum
-
 Prephenate
-
 ?
5.4.99.5 Chorismate Paraburkholderia phymatum DSM 17167 / CIP 108236 / LMG 21445 / STM815
-
 Prephenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.5 Paraburkholderia phymatum B2JYH9 i.e. Paraburkholderia phymatum, identified from root-nodule isolates from tropical legumes and is capable of symbiotic nitrogen fixation with the legumes Machaerium lunatum and Mimosa pudica
-
5.4.99.5 Paraburkholderia phymatum DSM 17167 / CIP 108236 / LMG 21445 / STM815 B2JYH9 i.e. Paraburkholderia phymatum, identified from root-nodule isolates from tropical legumes and is capable of symbiotic nitrogen fixation with the legumes Machaerium lunatum and Mimosa pudica
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.5 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)-R3 Rosetta by nickel affinity chromatography, gel filtration, and ultrafiltration Paraburkholderia phymatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.5 Chorismate
-
 Paraburkholderia phymatum Prephenate
-
 ?
5.4.99.5 Chorismate
-
 Paraburkholderia phymatum DSM 17167 / CIP 108236 / LMG 21445 / STM815 Prephenate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.99.5 More the enzyme is a chorismate mutases with AroQgamma topology Paraburkholderia phymatum

Synonyms

EC Number Synonyms Comment Organism
5.4.99.5 AroQ
-
 Paraburkholderia phymatum
5.4.99.5 Bphy_7813
-
 Paraburkholderia phymatum

General Information

EC Number General Information Comment Organism
5.4.99.5 evolution there are two classes of chorismate mutase: AroQ and AroH. The bacterial subclass AroQgamma has reported roles in virulence. Chorismate mutase from Burkholderia phymatum has the prototypical AroQgamma topology and retains the characteristic chorismate mutase active site Paraburkholderia phymatum
5.4.99.5 additional information the enzyme is a chorismate mutase with AroQgamma topology, enzyme structure analysis, enzyme topology, and conserved residues in the substrate-binding sites of chorismate mutase, overview Paraburkholderia phymatum