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Literature summary extracted from

  • Jaafar, N.R.; Littler, D.; Beddoe, T.; Rossjohn, J.; Illias, R.M.; Mahadi, N.M.; Mackeen, M.M.; Murad, A.M.; Abu Bakar, F.D.
    Crystal structure of fuculose aldolase from the antarctic psychrophilic yeast Glaciozyma antarctica PI12 (2016), Acta Crystallogr. Sect. F, 72, 831-839 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.17 expressed in Escherichia coli BL21(DE3) cells Glaciozyma antarctica

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.17 hanging drop vapor diffusion method, using 0.1 M Tris pH 8.0, 25% PEG 8000, 0.25 M MgCl2 Glaciozyma antarctica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.17 120000
-
 gel filtration Glaciozyma antarctica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.17 L-fuculose 1-phosphate Glaciozyma antarctica
-
 glycerone phosphate + (S)-lactaldehyde
-
 r
4.1.2.17 L-fuculose 1-phosphate Glaciozyma antarctica PI12
-
 glycerone phosphate + (S)-lactaldehyde
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.17 Glaciozyma antarctica
-
-
-
4.1.2.17 Glaciozyma antarctica PI12
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.17 Ni-NTA agarose column chromatography, HiTrap Q column chromatography, and Superdex 200 gel filtration Glaciozyma antarctica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.17 L-fuculose 1-phosphate
-
 Glaciozyma antarctica glycerone phosphate + (S)-lactaldehyde
-
 r
4.1.2.17 L-fuculose 1-phosphate
-
 Glaciozyma antarctica PI12 glycerone phosphate + (S)-lactaldehyde
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.2.17 homotetramer 4 * 30000, SDS-PAGE Glaciozyma antarctica
4.1.2.17 homotetramer 4 * 30688, calculated from amino acid sequence Glaciozyma antarctica

Synonyms

EC Number Synonyms Comment Organism
4.1.2.17 FucA
-
 Glaciozyma antarctica
4.1.2.17 fuculose aldolase
-
 Glaciozyma antarctica
4.1.2.17 fuculose-1-phosphate aldolase
-
 Glaciozyma antarctica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.2.17 Glaciozyma antarctica calculated from amino acid sequence
-
 6