EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.2.12 | expressed in Escherichia coli M15 (pREP4) cells | Staphylococcus aureus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.4.2.12 | sitting drop vapor diffusion method, using either 0.1 M HEPES pH 7.5, 20% (w/v) polyethylene glycol 10000 or 0.2 M NaCl, 0.1 M bis-Tris pH 6.5, 25% (w/v) polyethylene glycol 3350, at 25°C | Staphylococcus aureus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.4.2.12 | Mn2+ | dependent on | Staphylococcus aureus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.2.12 | 2-phospho-D-glycerate | Staphylococcus aureus | - |
3-phospho-D-glycerate | - |
? | |
5.4.2.12 | 2-phospho-D-glycerate | Staphylococcus aureus NCTC 8325 | - |
3-phospho-D-glycerate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.2.12 | Staphylococcus aureus | Q2G029 | - |
- |
5.4.2.12 | Staphylococcus aureus NCTC 8325 | Q2G029 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.4.2.12 | Ni-NTA Sepharose column chromatography and Superdex 200 gel filtration | Staphylococcus aureus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.2.12 | 2-phospho-D-glycerate | - |
Staphylococcus aureus | 3-phospho-D-glycerate | - |
? | |
5.4.2.12 | 2-phospho-D-glycerate | - |
Staphylococcus aureus NCTC 8325 | 3-phospho-D-glycerate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.2.12 | iPGM | - |
Staphylococcus aureus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.4.2.12 | additional information | the enzyme is not dependent from 2,3-bisphosphoglycerate | Staphylococcus aureus |