BRENDA - Enzyme Database

Expression, purification, crystallization and preliminary X-ray diffraction studies of phosphoglycerate mutase from Staphylococcus aureus NCTC8325

Roychowdhury, A.; Kundu, A.; Gujar, A.; Bose, M.; Das, A.K.; Acta Crystallogr. Sect. F 70, 53-56 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.2.12
expressed in Escherichia coli M15 (pREP4) cells
Staphylococcus aureus
Crystallization (Commentary)
EC Number
Crystallization
Organism
5.4.2.12
sitting drop vapor diffusion method, using either 0.1 M HEPES pH 7.5, 20% (w/v) polyethylene glycol 10000 or 0.2 M NaCl, 0.1 M bis-Tris pH 6.5, 25% (w/v) polyethylene glycol 3350, at 25°C
Staphylococcus aureus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.2.12
Mn2+
dependent on
Staphylococcus aureus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Staphylococcus aureus
-
3-phospho-D-glycerate
-
-
?
5.4.2.12
2-phospho-D-glycerate
Staphylococcus aureus NCTC 8325
-
3-phospho-D-glycerate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.12
Staphylococcus aureus
Q2G029
-
-
5.4.2.12
Staphylococcus aureus NCTC 8325
Q2G029
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.2.12
Ni-NTA Sepharose column chromatography and Superdex 200 gel filtration
Staphylococcus aureus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
746646
Staphylococcus aureus
3-phospho-D-glycerate
-
-
-
?
5.4.2.12
2-phospho-D-glycerate
-
746646
Staphylococcus aureus NCTC 8325
3-phospho-D-glycerate
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
the enzyme is not dependent from 2,3-bisphosphoglycerate
Staphylococcus aureus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
expressed in Escherichia coli M15 (pREP4) cells
Staphylococcus aureus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
the enzyme is not dependent from 2,3-bisphosphoglycerate
Staphylococcus aureus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.4.2.12
sitting drop vapor diffusion method, using either 0.1 M HEPES pH 7.5, 20% (w/v) polyethylene glycol 10000 or 0.2 M NaCl, 0.1 M bis-Tris pH 6.5, 25% (w/v) polyethylene glycol 3350, at 25°C
Staphylococcus aureus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.2.12
Mn2+
dependent on
Staphylococcus aureus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Staphylococcus aureus
-
3-phospho-D-glycerate
-
-
?
5.4.2.12
2-phospho-D-glycerate
Staphylococcus aureus NCTC 8325
-
3-phospho-D-glycerate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
Ni-NTA Sepharose column chromatography and Superdex 200 gel filtration
Staphylococcus aureus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
746646
Staphylococcus aureus
3-phospho-D-glycerate
-
-
-
?
5.4.2.12
2-phospho-D-glycerate
-
746646
Staphylococcus aureus NCTC 8325
3-phospho-D-glycerate
-
-
-
?