Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Huang, A.; Baker, E.; Loomes, K.
    Use of a novel microtitration protocol to obtain diffraction-quality crystals of 4-hydroxy-2-oxoglutarate aldolase from Bos taurus (2014), Acta Crystallogr. Sect. F, 70, 1546-1549 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.3.16 hamging drop vapor diffusion method, using 100 mM HEPES-NaOH pH 7.0, 200 mM Na2SO4, 22.5% (w/v) PEG 3350, and 20% (v/v) glycerol or 100 mM Tris-HCl pH 8.0 and 1.8 M ammonium sulfate Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.16 90000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.16 4-Hydroxy-2-oxoglutarate Bos taurus
-
 Pyruvate + glyoxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.16 Bos taurus Q0P5I5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.16 phenyl Sepharose column chromatography, Sepharose Q column chromatography, and Superdex 200 gel filtration Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.16 kidney
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.16 4-Hydroxy-2-oxoglutarate
-
 Bos taurus Pyruvate + glyoxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.16 ? x * 30000, SDS-PAGE Bos taurus
4.1.3.16 ? x * 32553, electrospray ionization mass spectrometry Bos taurus

Synonyms

EC Number Synonyms Comment Organism
4.1.3.16 hOGA
-
 Bos taurus