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Literature summary extracted from

  • Bashiri, G.; Johnston, J.M.; Evans, G.L.; Bulloch, E.M.; Goldstone, D.C.; Jirgis, E.N.; Kleinboelting, S.; Castell, A.; Ramsay, R.J.; Manos-Turvey, A.; Payne, R.J.; Lott, J.S.; Baker, E.N.
    Structure and inhibition of subunit I of the anthranilate synthase complex of Mycobacterium tuberculosis and expression of the active complex (2015), Acta Crystallogr. Sect. D, 71, 2297-2308 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.27 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.3.27 sitting drop vapor diffusion method, using 150 mM trisodium citrate pH 5.5, 0.75-1.5 M ammonium sulfate, 0.25-0.5 M lithium sulfate Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.27 3-(1-carboxyethoxy)-4,5-dihydroxybenzoate
-
 Mycobacterium tuberculosis
4.1.3.27 3-[(1-carboxylatoethenyl)oxy]-2-hydroxybenzoate
-
 Mycobacterium tuberculosis
4.1.3.27 3-[(1-carboxylatoethenyl)oxy]benzoate
-
 Mycobacterium tuberculosis
4.1.3.27 3-[(2-carboxyprop-2-en-1-yl)oxy]-4,5-dihydroxybenzoate
-
 Mycobacterium tuberculosis
4.1.3.27 3-[[(1E)-1-carboxylatobut-1-en-1-yl]oxy]-2-hydroxybenzoate
-
 Mycobacterium tuberculosis
4.1.3.27 3-[[(1E)-1-carboxylatoprop-1-en-1-yl]oxy]-2-hydroxybenzoate
-
 Mycobacterium tuberculosis
4.1.3.27 3-[[(E)-1-carboxylato-2-phenylethenyl]oxy]-2-hydroxybenzoate
-
 Mycobacterium tuberculosis
4.1.3.27 tryptophan allosteric inhibition Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.27 0.015
-
 chorismate at pH 8.0 and 25°C Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.27 chorismate + L-glutamine Mycobacterium tuberculosis
-
 anthranilate + pyruvate + L-glutamate
-
 ?
4.1.3.27 chorismate + L-glutamine Mycobacterium tuberculosis CDC 1551
-
 anthranilate + pyruvate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.27 Mycobacterium tuberculosis P9WFX2
-
-
4.1.3.27 Mycobacterium tuberculosis CDC 1551 P9WFX2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.27 Ni-NTA column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.27 chorismate + L-glutamine
-
 Mycobacterium tuberculosis anthranilate + pyruvate + L-glutamate
-
 ?
4.1.3.27 chorismate + L-glutamine
-
 Mycobacterium tuberculosis CDC 1551 anthranilate + pyruvate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.27 ? x * 54000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
4.1.3.27 AS-I
-
 Mycobacterium tuberculosis
4.1.3.27 TrpE
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.27 0.26
-
 chorismate at pH 8.0 and 25°C Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.1.3.27 0.0063
-
 at pH 8.0 and 25°C Mycobacterium tuberculosis tryptophan