Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Williamson, A.; Rothweiler, U.; Leiros, H.K.
    Enzyme-adenylate structure of a bacterial ATP-dependent DNA ligase with a minimized DNA-binding surface (2014), Acta Crystallogr. Sect. D, 70, 3043-3056 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 expressed in Escherichia coli BL21(DE3)Star cells Psychromonas sp. SP041

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.5.1.1 hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.4, 1.6-1.9 M ammonium sulfate Psychromonas sp. SP041

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.1 additional information no inhibition with ATP concentrations up to 0.5 mM Psychromonas sp. SP041
6.5.1.1 NaCl NaCl concentrations above 100 mM have an inhibitory effect Psychromonas sp. SP041

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.1 0.000124
-
 (nicked double-stranded DNA)n at 30°C, pH not specified in the publication Psychromonas sp. SP041
6.5.1.1 0.0038
-
 ATP at 30°C, pH not specified in the publication Psychromonas sp. SP041

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Mg2+ MgCl2 is required for enzyme activity, with an optimal concentration between 1 and 5 mM Psychromonas sp. SP041

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m Psychromonas sp. SP041
-
 (deoxyribonucleotide)n+m + AMP + diphosphate
-
 ?
6.5.1.1 ATP + [DNA ligase]-L-lysine Psychromonas sp. SP041
-
 [DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Psychromonas sp. SP041 A0A0A6YVN6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1
-
 Psychromonas sp. SP041

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m
-
 Psychromonas sp. SP041 (deoxyribonucleotide)n+m + AMP + diphosphate
-
 ?
6.5.1.1 ATP + (nicked double-stranded DNA)n + (nicked double-stranded DNA)m
-
 Psychromonas sp. SP041 AMP + diphosphate + (nicked double-stranded DNA)n+m
-
 ?
6.5.1.1 ATP + [DNA ligase]-L-lysine
-
 Psychromonas sp. SP041 [DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
 ?
6.5.1.1 additional information in addition to sealing a singly nicked fully matched double-stranded DNA substrate, the enzyme is also able to ligate a cohesive-ended double-stranded break and a single nick with a 1 base pair mismatch at the 3'-OH position of the nick, albeit with reduced efficiency. Ligation of a blunt-end double-stranded break and a 1 nucleotide gapped substrate is also detected when enzyme concentrations and extended incubation times are used; however, no activity is seen with a nonphosphorylated 5'-nicked strand or single-stranded DNA Psychromonas sp. SP041 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 ADL
-
 Psychromonas sp. SP041
6.5.1.1 ATP-dependent DNA ligase
-
 Psychromonas sp. SP041

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5.1.1 0.05
-
 (nicked double-stranded DNA)n at 30°C, pH not specified in the publication Psychromonas sp. SP041