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Literature summary extracted from

  • Awwad, K.; Desai, A.; Smith, C.; Sommerhalter, M.
    Structural and functional characterization of a noncanonical nucleoside triphosphate pyrophosphatase from Thermotoga maritima (2013), Acta Crystallogr. Sect. D, 69, 184-193 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.66 protein X-ray structure of the enzyme with bound IMP is obtained at 2.15 A resolution and compare it with structures of unliganded TM0159 and other noncanonical NTPases Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.66 0.06
-
 ITP pH 9.0, 50°C Thermotoga maritima
3.6.1.66 0.07
-
 dITP pH 9.0, 50°C Thermotoga maritima
3.6.1.66 0.11
-
 XTP pH 9.0, 50°C Thermotoga maritima
3.6.1.66 0.45
-
 dITP pH 9.0, 80°C Thermotoga maritima
3.6.1.66 0.51
-
 ITP pH 9.0, 80°C Thermotoga maritima
3.6.1.66 1.69
-
 XTP pH 9.0, 80°C Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.66 Mg2+ absolute requirement for Mg2+. At 10 mM Mg2+ concentration, the enzyme reaches a plateau of optimum activity. Other divalent and monovalent cations barely support the catalytic activity of TM0159. Samples containing up to 100 mM Mn2+, Zn2+, Co2+, Cu2+, Na+, K+ or Li+ show less than 10% of the activity measured at 10 mM Mg2+ Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.66 Thermotoga maritima Q9WY06
-
-
3.6.1.66 Thermotoga maritima DSM 3109 Q9WY06
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.66 dITP + H2O ITP and dITP are slightly preferred over XTP Thermotoga maritima dTDP + phosphate
-
 ?
3.6.1.66 dITP + H2O ITP and dITP are slightly preferred over XTP Thermotoga maritima DSM 3109 dTDP + phosphate
-
 ?
3.6.1.66 ITP + H2O ITP and dITP are slightly preferred over XTP Thermotoga maritima IDP + phosphate
-
 ?
3.6.1.66 ITP + H2O ITP and dITP are slightly preferred over XTP Thermotoga maritima DSM 3109 IDP + phosphate
-
 ?
3.6.1.66 XTP + H2O ITP and dITP are slightly preferred over XTP Thermotoga maritima XDP + phosphate
-
 ?
3.6.1.66 XTP + H2O ITP and dITP are slightly preferred over XTP Thermotoga maritima DSM 3109 XDP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.66 tetramer crystal structure shows a tetramer with two possible dimer interfaces Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
3.6.1.66 TM0159
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.6.1.66 22
-
 no significant activity is detected at room temperature Thermotoga maritima
3.6.1.66 50 80 assay at Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.66 2.7
-
 XTP pH 9.0, 50°C Thermotoga maritima
3.6.1.66 3.9
-
 ITP pH 9.0, 50°C Thermotoga maritima
3.6.1.66 5.46
-
 dITP pH 9.0, 50°C Thermotoga maritima
3.6.1.66 13.87
-
 ITP pH 9.0, 80°C Thermotoga maritima
3.6.1.66 18.68
-
 dITP pH 9.0, 80°C Thermotoga maritima
3.6.1.66 22.08
-
 XTP pH 9.0, 80°C Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.66 9
-
 preference for alkaline conditions Thermotoga maritima

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.66 13.1
-
 XTP pH 9.0, 80°C Thermotoga maritima
3.6.1.66 24.5
-
 XTP pH 9.0, 50°C Thermotoga maritima
3.6.1.66 27.2
-
 ITP pH 9.0, 80°C Thermotoga maritima
3.6.1.66 41.5
-
 dITP pH 9.0, 80°C Thermotoga maritima
3.6.1.66 65
-
 ITP pH 9.0, 50°C Thermotoga maritima
3.6.1.66 78
-
 dITP pH 9.0, 50°C Thermotoga maritima