Literature summary extracted from

Black, W.B.; Zhang, L.; Kamoku, C.; Liao, J.C.; Li, H.
Rearrangement of coenzyme A-acylated carbon chain enables synthesis of isobutanol via a novel pathway in Ralstonia eutropha (2018), ACS Synth. Biol., 7, 794-800 .

Application

EC Number	Application	Comment	Organism
5.4.99.13	biofuel production	the production of isobutanol, a branched-chain alcohol that can be used as a gasoline substitute, using a CoA-dependent pathway in recombinant Ralstonia eutropha strain H16. The designed pathway involves isobutyryl-CoA mutase activity. The engineered strain produces about 30 mg/l isobutanol from fructose	Cupriavidus necator
5.4.99.13	synthesis	the production of isobutanol, a branched-chain alcohol that can be used as a gasoline substitute, using a CoA-dependent pathway in recombinant Ralstonia eutropha strain H16. The designed pathway involves isobutyryl-CoA mutase. The engineered strain produces about 30 mg/l isobutanol from fructose. The carbon skeleton rearrangement chemistry demonstrated may be used to expand the range of the chemicals accessible with CoA-dependent pathways	Cupriavidus necator

Protein Variants

EC Number	Protein Variants	Comment	Organism
5.4.99.13	additional information	the production of isobutanol, a branched-chain alcohol that can be used as a gasoline substitute, using a CoA-dependent pathway in recombinant Ralstonia eutropha strain H16. The designed pathway is constituted of three modules: chain elongation, rearrangement, and modification. First, the chain elongation and modification modules are integrated and optimized, esulting in the production of about 200 mg/l n-butanol from fructose or 30 mg/L from formate by engineered Ralstonia eutropha. Subsequently, the rearrangement module is incorporated, featuring native isobutyryl-CoA mutase in Ralstonia eutropha. The engineered strain produces about 30 mg/l isobutanol from fructose. The demonstrated carbon skeleton rearrangement chemistry may be used to expand the range of the chemicals accessible with CoA-dependent pathways	Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5.4.99.13	2-methylpropanoyl-CoA	Cupriavidus necator	-	butanoyl-CoA	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
5.4.99.13	Cupriavidus necator	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5.4.99.13	2-methylpropanoyl-CoA	-	Cupriavidus necator	butanoyl-CoA	-	?

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Release 2023.1 (January 2023)