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Literature summary extracted from

  • Harrington, L.B.; Jha, R.K.; Kern, T.L.; Schmidt, E.N.; Canales, G.M.; Finney, K.B.; Koppisch, A.T.; Strauss, C.E.; Fox, D.T.
    Rapid thermostabilization of Bacillus thuringiensis serovar Konkukian 97-27 dehydroshikimate dehydratase through a structure-based enzyme design and whole cell activity assay (2017), ACS Synth. Biol., 6, 120-129 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.118 in vivo overexpression of enzyme mutant Mut1 in Escherichiaxa0coli results in a about 60fold increase in functional enzyme when compared to the wild-type enzyme under the identical expression conditions, and 80-120% increase in DHB accumulation in the media Bacillus thuringiensis

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.118 additional information gene asbF is unstable at 37°C, structure-based design to identify stabilizing mutations and creation a combinatorial library based upon predicted mutations at specific locations on the enzyme surface. A diversified asbF library (with about 2000 variants) is expressed in Escherichiaxa0coli harboring a GFP reporter system linked to the product of AsbF activity (3,4-dihydroxybenzoate, DHB) Bacillus thuringiensis
4.2.1.118 T61N/H135Y/H257P random mutagenesis, triple AsbF mutant Mut1, half-life of Mut1 at 37°C is over 10fold higher compared to wild-type AsbF. The second-order rate constants for both wild-type AsbF and Mut1 are approximately equal, thus demonstrating protein thermostability does not come at the expense of enzyme thermophilicity Bacillus thuringiensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.118 3-dehydro-shikimate Bacillus thuringiensis
-
 protocatechuate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.118 Bacillus thuringiensis A0A1B1L4G3 serovar Konkukian 97-27
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.118 3-dehydro-shikimate
-
 Bacillus thuringiensis protocatechuate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.118 AsbF
-
 Bacillus thuringiensis
4.2.1.118 BT246_21860
-
 Bacillus thuringiensis
4.2.1.118 dehydroshikimate dehydratase
-
 Bacillus thuringiensis
4.2.1.118 DHSase
-
 Bacillus thuringiensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.118 37
-
 recombinant wild-type enzyme AsbF, t1/2 is 169 min Bacillus thuringiensis