EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.3.43 | expressed in Escherichia coli BL21(DE3)RIL-CodonPlus cells | Diaporthe amygdali |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.2.3.43 | sitting drop vapor diffusion method | Diaporthe amygdali |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.3.43 | D474A | partially inactivated mutant | Diaporthe amygdali |
4.2.3.43 | D92A | the mutant enzxme does not generate any cyclic products yet retains geranylgeranyl diphosphate synthase activity | Diaporthe amygdali |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.43 | 0.00014 | - |
(2E,6E)-farnesyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali | |
4.2.3.43 | 0.00063 | - |
geranylgeranyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali | |
4.2.3.43 | 0.0092 | - |
geranyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.3.43 | Mg2+ | essential | Diaporthe amygdali |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.3.43 | 502000 | - |
gel filtration | Diaporthe amygdali |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.43 | geranylgeranyl diphosphate | Diaporthe amygdali | - |
fusicocca-2,10(14)-diene + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.43 | Diaporthe amygdali | A2PZA5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.3.43 | - |
Diaporthe amygdali |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.43 | (2E,6E)-farnesyl diphosphate | - |
Diaporthe amygdali | (E,E)-farnesol + (E)-nerolidol + farnesene + diphosphate | - |
? | |
4.2.3.43 | geranyl diphosphate | - |
Diaporthe amygdali | geraniol + linalool + diphosphate | - |
? | |
4.2.3.43 | geranylgeranyl diphosphate | - |
Diaporthe amygdali | fusicocca-2,10(14)-diene + diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.3.43 | homohexamer | small-angle X-ray scattering | Diaporthe amygdali |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.3.43 | fusicoccadiene synthase | - |
Diaporthe amygdali |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.43 | 0.021 | - |
geranylgeranyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali | |
4.2.3.43 | 0.027 | - |
(2E,6E)-farnesyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali | |
4.2.3.43 | 0.036 | - |
geranyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.43 | 3.9 | - |
geranyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali | |
4.2.3.43 | 33 | - |
geranylgeranyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali | |
4.2.3.43 | 190 | - |
(2E,6E)-farnesyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali |