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Literature summary extracted from

  • Chen, M.; Chou, W.K.; Toyomasu, T.; Cane, D.E.; Christianson, D.W.
    Structure and function of fusicoccadiene synthase, a hexameric bifunctional diterpene synthase (2016), ACS Chem. Biol., 11, 889-899 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.43 expressed in Escherichia coli BL21(DE3)RIL-CodonPlus cells Diaporthe amygdali

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.3.43 sitting drop vapor diffusion method Diaporthe amygdali

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.43 D474A partially inactivated mutant Diaporthe amygdali
4.2.3.43 D92A the mutant enzxme does not generate any cyclic products yet retains geranylgeranyl diphosphate synthase activity Diaporthe amygdali

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.43 0.00014
-
(2E,6E)-farnesyl diphosphate pH and temperature not specified in the publication Diaporthe amygdali
4.2.3.43 0.00063
-
geranylgeranyl diphosphate pH and temperature not specified in the publication Diaporthe amygdali
4.2.3.43 0.0092
-
geranyl diphosphate pH and temperature not specified in the publication Diaporthe amygdali

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.43 Mg2+ essential Diaporthe amygdali

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.43 502000
-
gel filtration Diaporthe amygdali

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.43 geranylgeranyl diphosphate Diaporthe amygdali
-
fusicocca-2,10(14)-diene + diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.43 Diaporthe amygdali A2PZA5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.43
-
Diaporthe amygdali

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.43 (2E,6E)-farnesyl diphosphate
-
Diaporthe amygdali (E,E)-farnesol + (E)-nerolidol + farnesene + diphosphate
-
?
4.2.3.43 geranyl diphosphate
-
Diaporthe amygdali geraniol + linalool + diphosphate
-
?
4.2.3.43 geranylgeranyl diphosphate
-
Diaporthe amygdali fusicocca-2,10(14)-diene + diphosphate
-
?

Subunits

EC Number Subunits Comment Organism
4.2.3.43 homohexamer small-angle X-ray scattering Diaporthe amygdali

Synonyms

EC Number Synonyms Comment Organism
4.2.3.43 fusicoccadiene synthase
-
Diaporthe amygdali

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.43 0.021
-
geranylgeranyl diphosphate pH and temperature not specified in the publication Diaporthe amygdali
4.2.3.43 0.027
-
(2E,6E)-farnesyl diphosphate pH and temperature not specified in the publication Diaporthe amygdali
4.2.3.43 0.036
-
geranyl diphosphate pH and temperature not specified in the publication Diaporthe amygdali

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.43 3.9
-
geranyl diphosphate pH and temperature not specified in the publication Diaporthe amygdali
4.2.3.43 33
-
geranylgeranyl diphosphate pH and temperature not specified in the publication Diaporthe amygdali
4.2.3.43 190
-
(2E,6E)-farnesyl diphosphate pH and temperature not specified in the publication Diaporthe amygdali