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Literature summary extracted from

  • Lin, F.; Ferguson, K.L.; Boyer, D.R.; Lin, X.N.; Marsh, E.N.
    Isofunctional enzymes PAD1 and UbiX catalyze formation of a novel cofactor required by ferulic acid decarboxylase and 4-hydroxy-3-polyprenylbenzoic acid decarboxylase (2015), ACS Chem. Biol., 10, 1137-1144 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.102 expression in Escherichia coli Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.102 0.11
-
 ferulate pH 7.0, 25°C Saccharomyces cerevisiae
4.1.1.102 0.18
-
 4-coumarate pH 7.0, 25°C Saccharomyces cerevisiae
4.1.1.102 0.18
-
 trans-cinnamate pH 7.0, 25°C Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.102 480000
-
 PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.102 Saccharomyces cerevisiae Q03034
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.102 4-coumarate
-
 Saccharomyces cerevisiae 4-vinylphenol + CO2
-
 ?
4.1.1.102 ferulate
-
 Saccharomyces cerevisiae 4-vinylguaiacol + CO2
-
 ?
4.1.1.102 trans-cinnamate
-
 Saccharomyces cerevisiae styrene + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.102 oligomer x * 57898, mass spectrometry, x * 57898, calculated from sequence, recombinant His-tagged protein Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.102 1.5
-
 ferulate pH 7.0, 25°C Saccharomyces cerevisiae
4.1.1.102 3.8
-
 trans-cinnamate pH 7.0, 25°C Saccharomyces cerevisiae
4.1.1.102 4.6
-
 4-coumarate pH 7.0, 25°C Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.102 FMN a modified form of FMN acts as cofactor Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
4.1.1.102 physiological function protein PAD1 catalyzes the formation of a diffusible cofactor required by FDC for decarboxylase activity. Escherichia coli protein UbiX can also activate FDC Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.102 13.6
-
 ferulate pH 7.0, 25°C Saccharomyces cerevisiae
4.1.1.102 21
-
 trans-cinnamate pH 7.0, 25°C Saccharomyces cerevisiae
4.1.1.102 25.5
-
 4-coumarate pH 7.0, 25°C Saccharomyces cerevisiae