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Literature summary extracted from

  • Karuppiah, V.; Ranaghan, K.E.; Leferink, N.G.H.; Johannissen, L.O.; Shanmugam, M.; Ni Cheallaigh, A.; Bennett, N.J.; Kearsey, L.J.; Takano, E.; Gardiner, J.M.; van der Kamp, M.W.; Hay, S.; Mulholland, A.J.; Leys, D.; Scrutton, N.S.
    Structural basis of catalysis in the bacterial monoterpene synthases linalool synthase and 1,8-cineole synthase (2017), ACS Catal., 7, 6268-6282 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.25 expressed in Escherichia coli ArcticExpress (DE3) cells Streptomyces clavuligerus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.3.25 vapor diffusion method, using 40% v/v glycerol and 20% w/v PEG 4000 Streptomyces clavuligerus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.25 Mg2+ contains 3 Mg2+ ions Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.25 geranyl diphosphate + H2O Streptomyces clavuligerus
-
 (3S)-linalool + diphosphate
-
 ?
4.2.3.25 geranyl diphosphate + H2O Streptomyces clavuligerus ATCC 27064
-
 (3S)-linalool + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.25 Streptomyces clavuligerus
-
-
-
4.2.3.25 Streptomyces clavuligerus ATCC 27064
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.25 HisTrap column chromatography and Superdex S75 gel filtration Streptomyces clavuligerus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.25 (2E,6E)-farnesyl diphosphate + H2O
-
 Streptomyces clavuligerus trans-nerolidol + diphosphate
-
 ?
4.2.3.25 (2E,6E)-farnesyl diphosphate + H2O
-
 Streptomyces clavuligerus ATCC 27064 trans-nerolidol + diphosphate
-
 ?
4.2.3.25 2-fluorogeranyl diphosphate + H2O
-
 Streptomyces clavuligerus ?
-
 ?
4.2.3.25 2-fluorogeranyl diphosphate + H2O
-
 Streptomyces clavuligerus ATCC 27064 ?
-
 ?
4.2.3.25 2-fluoroneryl diphosphate + H2O
-
 Streptomyces clavuligerus ?
-
 ?
4.2.3.25 2-fluoroneryl diphosphate + H2O
-
 Streptomyces clavuligerus ATCC 27064 ?
-
 ?
4.2.3.25 geranyl diphosphate + H2O
-
 Streptomyces clavuligerus (3S)-linalool + diphosphate
-
 ?
4.2.3.25 geranyl diphosphate + H2O
-
 Streptomyces clavuligerus ATCC 27064 (3S)-linalool + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.25 homodimer x-ray crystallography Streptomyces clavuligerus

Synonyms

EC Number Synonyms Comment Organism
4.2.3.25 bLINS
-
 Streptomyces clavuligerus
4.2.3.25 linalool synthase
-
 Streptomyces clavuligerus