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Literature summary extracted from

  • Subrizi, F.; Crucianelli, M.; Grossi, V.; Passacantando, M.; Botta, G.; Antiochia, R.; Saladino, R.
    Versatile and efficient immobilization of 2-deoxyribose-5-phosphate aldolase (DERA) on multiwalled carbon nanotubes (2014), ACS Catal., 4, 3059-3068 .
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.1.2.4 the enzyme directly immobilized through ionic exchange interactions on oxidized multiwalled carbon nanotubes is stable with a high tolerance to acetaldehyde, and maintains its activity for several days, being reused for five runs Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.4 0.34
-
2-deoxy-D-ribose 5-phosphate pH 7.5, 25°C, enzyme directly immobilized through ionic exchange interactions on oxidized multiwalled carbon nanotubes Escherichia coli
4.1.2.4 0.55
-
2-deoxy-D-ribose 5-phosphate pH 7.5, 25°C, soluble enzyme Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.4 Escherichia coli P0A6L0
-
-

Storage Stability

EC Number Storage Stability Organism
4.1.2.4 25°C, up to 15 days, the enzyme directly immobilized through ionic exchange interactions on oxidized multiwalled carbon nanotubes retains almost the full activity. The soluble enzyme shows a decrease of activity after the first 5 days, thereby reaching a value of ca. 80% of residual activity after 15 days Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.4 2-deoxy-D-ribose 5-phosphate
-
Escherichia coli D-glyceraldehyde 3-phosphate + acetaldehyde
-
?
4.1.2.4 acetaldehyde + acetaldehyde enzyme directly immobilized through ionic exchange interactions on oxidized multiwalled carbon nanotubes catalyzes the reaction of acetaldehyde alone (self-condensation) or in the presence of chloro-acetaldehyde. The corresponding cyclic lactols are obtained in higher yield than with the native enzyme Escherichia coli ?
-
?
4.1.2.4 chloroacetaldehyde + acetaldehyde enzyme directly immobilized through ionic exchange interactions on oxidized multiwalled carbon nanotubes catalyzes the reaction of acetaldehyde alone (self-condensation) or in the presence of chloro-acetaldehyde. The corresponding cyclic lactols are obtained in higher yield than with the native enzyme Escherichia coli ?
-
?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.4 2-Deoxyribose-5-phosphate aldolase
-
Escherichia coli
4.1.2.4 DERA
-
Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.4 25
-
assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.4 7.5
-
assay at Escherichia coli