Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kusaykin, M.I.; Belik, A.A.; Kovalchuk, S.N.; Dmitrenok, P.S.; Rasskazov, V.A.; Isakov, V.V.; Zvyagintseva, T.N.
    A new recombinant endo-1,3-beta-D-glucanase from the marine bacterium Formosa algae KMM 3553 enzyme characteristics and transglycosylation products analysis (2017), World J. Microbiol. Biotechnol., 33, 40 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 expressed in Escherichia coli Rosetta (DE3) cells Formosa algae
3.2.1.39 expression in Escherichia coli Formosa algae

General Stability

EC Number General Stability Organism
3.2.1.39 62% of activity is retained at 2.0 M NaCl Formosa algae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 0.388
-
 Laminarin at pH 5.4 and 37°C Formosa algae
3.2.1.39 0.39
-
 Laminarin pH 6.0, 37°C Formosa algae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Formosa algae
-
-
-
3.2.1.39 Formosa algae A0A0B5GQL6
-
-
3.2.1.39 Formosa algae KMM 3553
-
-
-
3.2.1.39 Formosa algae KMM 3553 A0A0B5GQL6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 DEAE-cellulose column chromatography, Ni-NTA agarose column chromatography, and Sephacryl S-100 gel filtration Formosa algae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 24
-
 pH 6.0 37°C Formosa algae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 carboxymethyl-pachyman + H2O 1.7% activity compared to laminarin Formosa algae ?
-
 ?
3.2.1.39 laminarin + H2O 100% activity Formosa algae ?
-
 ?
3.2.1.39 laminarin + H2O 100% activity Formosa algae KMM 3553 ?
-
 ?
3.2.1.39 laminarin + H2O laminarin from Saccharina cichorioides with molecular weight of 3.5 kDa Formosa algae laminaritrisaccharides + laminaritetrasaccharides
-
 ?
3.2.1.39 laminarin + H2O laminarin from Saccharina cichorioides with molecular weight of 3.5 kDa Formosa algae KMM 3553 laminaritrisaccharides + laminaritetrasaccharides
-
 ?
3.2.1.39 lichenan + H2O 1.1% activity compared to laminarin Formosa algae ?
-
 ?
3.2.1.39 lichenan + H2O 1.1% activity compared to laminarin Formosa algae KMM 3553 ?
-
 ?
3.2.1.39 additional information enzyme catalyzes transfer of the glyconic part of a substrate molecule on methyl-beta-D-xylopyranoside, glycerol and methyl-alpha-D-glucopyranoside. Low activity on yeast glucan, no substrates: pachyman and aubasidan, pustulan, pullulan, amylopectin, and carboxymethylcellulose Formosa algae ?
-
 ?
3.2.1.39 additional information the enzyme catalyzes transfer of glyconic part of substrate molecule on methyl-beta-D-xylopyranoside, glycerol and methyl-alpha-D-glucopyranoside Formosa algae ?
-
 ?
3.2.1.39 additional information the enzyme has no ability to degrade pachiman, aubasidan, pustulan, pullulan, amylopectin, and carboxymethyl-cellulose Formosa algae ?
-
 ?
3.2.1.39 additional information the enzyme catalyzes transfer of glyconic part of substrate molecule on methyl-beta-D-xylopyranoside, glycerol and methyl-alpha-D-glucopyranoside Formosa algae KMM 3553 ?
-
 ?
3.2.1.39 additional information the enzyme has no ability to degrade pachiman, aubasidan, pustulan, pullulan, amylopectin, and carboxymethyl-cellulose Formosa algae KMM 3553 ?
-
 ?
3.2.1.39 additional information enzyme catalyzes transfer of the glyconic part of a substrate molecule on methyl-beta-D-xylopyranoside, glycerol and methyl-alpha-D-glucopyranoside. Low activity on yeast glucan, no substrates: pachyman and aubasidan, pustulan, pullulan, amylopectin, and carboxymethylcellulose Formosa algae KMM 3553 ?
-
 ?
3.2.1.39 periodate-oxidized laminarin + H2O
-
 Formosa algae ?
-
 ?
3.2.1.39 periodate-oxidized laminarin + H2O 98% activity compared to laminarin Formosa algae ?
-
 ?
3.2.1.39 periodate-oxidized laminarin + H2O 98% activity compared to laminarin Formosa algae KMM 3553 ?
-
 ?
3.2.1.39 periodate-oxidized laminarin + H2O
-
 Formosa algae KMM 3553 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 61000, SDS-PAGE Formosa algae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 endo-1,3-beta-D-glucanase
-
 Formosa algae
3.2.1.39 Gfa
-
 Formosa algae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 45
-
-
 Formosa algae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.39 35 50 the enzyme shows about 50% activity at 30°C, about 60% activity at 35°C, about 80% activity at 40°C, 100% activity at 45°C, about 30% activity at 50°C, and no activity at 55°C and above Formosa algae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 45
-
 half-life 20 min Formosa algae
3.2.1.39 45 55 the half-life period at 45°C is 20 min, and complete inactivation happens at 55°C within 10 min Formosa algae
3.2.1.39 55
-
 10 min, complete loss of activity Formosa algae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 5.5
-
-
 Formosa algae
3.2.1.39 5.5 6
-
 Formosa algae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.39 4 8 the enzyme shows more than 60% activity between pH 4.0 and 8.0 Formosa algae