Literature summary extracted from
Zhu, Y.; Wang, G.; Ni, H.; Xiao, A.; Cai, H.
Cloning and characterization of a new manganese superoxide dismutase from deep-sea thermophile Geobacillus sp. EPT3 (2014), World J. Microbiol. Biotechnol., 30, 1347-1357 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.15.1.1 |
DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
Geobacillus sp. EPT3 |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.15.1.1 |
Ba2+ |
inhibits at 1 mM |
Geobacillus sp. EPT3 |
|
1.15.1.1 |
Ca2+ |
inhibits at 1 mM |
Geobacillus sp. EPT3 |
|
1.15.1.1 |
Co2+ |
inhibits at 1 mM |
Geobacillus sp. EPT3 |
|
1.15.1.1 |
Cu2+ |
inhibits at 1 mM |
Geobacillus sp. EPT3 |
|
1.15.1.1 |
Fe2+ |
inhibits at 1 mM |
Geobacillus sp. EPT3 |
|
1.15.1.1 |
Mg2+ |
inhibits at 1 mM |
Geobacillus sp. EPT3 |
|
1.15.1.1 |
additional information |
insensitivity of the recombinant enzyme to both KCN and H2O2. The enzyme shows a relatively good tolerance to some inhibitors, detergents, and denaturants, such as 2-mercaptoethanol, dithiothreitol, phenylmethylsulfonyl fluoride, Chaps, Triton X-100, urea, and guanidine hydrochloride |
Geobacillus sp. EPT3 |
|
1.15.1.1 |
Ni2+ |
inhibits slightly at 1 mM |
Geobacillus sp. EPT3 |
|
1.15.1.1 |
Zn2+ |
inhibits at 1 mM |
Geobacillus sp. EPT3 |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.15.1.1 |
Mn2+ |
a Mn-SOD, Mn2+ activate the enzyme at 1 mM, the manganese-binding sites are conserved in the sequence involving residues His260, His308, Asp392, and His396 |
Geobacillus sp. EPT3 |
|
1.15.1.1 |
additional information |
the Fe2+-reconstituted SOD is inactive |
Geobacillus sp. EPT3 |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.15.1.1 |
130700 |
- |
recombinant His-tagged enzyme, gel filtration |
Geobacillus sp. EPT3 |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.15.1.1 |
2 superoxide + 2 H+ |
Geobacillus sp. EPT3 |
- |
O2 + H2O2 |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.15.1.1 |
Geobacillus sp. EPT3 |
T1T1K2 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.15.1.1 |
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis |
Geobacillus sp. EPT3 |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.15.1.1 |
1204 |
- |
purified recombinant His-tagged enzyme, pH 7.8, 25°C |
Geobacillus sp. EPT3 |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.15.1.1 |
2 superoxide + 2 H+ |
- |
Geobacillus sp. EPT3 |
O2 + H2O2 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.15.1.1 |
homodimer |
2 * 59000, recombinant His-tagged enzyme, SDS-PAGE |
Geobacillus sp. EPT3 |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.15.1.1 |
manganese-containing SOD |
- |
Geobacillus sp. EPT3 |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.15.1.1 |
25 |
- |
assay at |
Geobacillus sp. EPT3 |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
1.15.1.1 |
50 |
- |
purified recombinant enzyme, 1 h, stable at |
Geobacillus sp. EPT3 |
1.15.1.1 |
90 |
- |
purified recombinant enzyme, 57% activity remaining after incubation for 1 h |
Geobacillus sp. EPT3 |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.15.1.1 |
8 |
- |
- |
Geobacillus sp. EPT3 |
pH Stability
EC Number |
pH Stability |
pH Stability Maximum |
Comment |
Organism |
---|
1.15.1.1 |
5 |
11 |
purified recombinant enzyme, 1 h, highly stable at |
Geobacillus sp. EPT3 |