EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.3.3 | - |
Mycobacterium tuberculosis |
6.3.3.3 | - |
Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
6.3.3.3 | in complex with CTP, to 2.3 A resolution. The nucleoside base is stabilized in its pocket through hydrogen-bonding interactions with the protein backbone, rather than amino acid side chains. DTBS could utilise ATP, CTP, GTP, ITP, TTP, or UTP with similar Km and kcat values | Mycobacterium tuberculosis |
6.3.3.3 | in complex with CTP, to 2.3 A resolution. The nucleoside base is stabilized in its pocket through hydrogen-bonding interactions with the protein backbone, rather than amino acid side chains. DTBS could utilise ATP, CTP, GTP, ITP, TTP, or UTP with similar Km and kcat values | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.3.3 | G169N | Km value for (7R, 8S)-7,8-diaminopelargonic acid similar to wild-type, 3fold increase in Km value for carbonate | Mycobacterium tuberculosis |
6.3.3.3 | N175A | 2.7fold decrease in Km value for CTP | Escherichia coli |
6.3.3.3 | N175G | no significant effects on Km value for ATP, CTP | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.3.3 | ATP | - |
Escherichia coli | |
6.3.3.3 | ATP | - |
Mycobacterium tuberculosis | |
6.3.3.3 | CTP | - |
Escherichia coli | |
6.3.3.3 | CTP | - |
Mycobacterium tuberculosis | |
6.3.3.3 | GTP | - |
Escherichia coli | |
6.3.3.3 | GTP | - |
Mycobacterium tuberculosis | |
6.3.3.3 | ITP | - |
Mycobacterium tuberculosis | |
6.3.3.3 | TTP | - |
Mycobacterium tuberculosis | |
6.3.3.3 | UTP | - |
Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.3.3 | 0.01 | - |
ATP | wild-type, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 0.012 | - |
ATP | mutant N175A, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 0.015 | - |
(7R,8S)-7,8-diaminopelargonic acid | wild-type, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 0.015 | - |
(7R,8S)-7,8-diaminopelargonic acid | mutant G169N, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 0.017 | - |
ATP | mutant N175G, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 0.018 | - |
ITP | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 0.018 | - |
(7R,8S)-7,8-diaminopelargonic acid | mutant N175G, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 0.019 | - |
(7R,8S)-7,8-diaminopelargonic acid | mutant N175A, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 0.022 | - |
ATP | mutant G169N, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 0.023 | - |
TTP | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 0.025 | - |
CTP | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 0.026 | - |
GTP | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 0.026 | - |
UTP | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 0.03 | - |
ATP | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 0.03 | - |
(7R,8S)-7,8-diaminopelargonic acid | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 0.045 | - |
CTP | mutant G169N, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 0.101 | - |
GTP | mutant G169N, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 0.384 | - |
NaHCO3 | mutant N175G, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 0.391 | - |
CTP | mutant N175A, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 0.43 | - |
NaHCO3 | mutant N175A, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 0.6 | - |
NaHCO3 | wild-type, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 0.869 | - |
CTP | mutant N175G, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 1.09 | - |
CTP | wild-type, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 1.09 | - |
GTP | mutant N175A, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 1.387 | - |
GTP | mutant N175G, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 1.56 | - |
GTP | wild-type, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 3.074 | - |
NaHCO3 | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 10.385 | - |
NaHCO3 | mutant G169N, pH 7.5, 37°C | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.3.3 | Escherichia coli | P13000 | - |
- |
6.3.3.3 | Mycobacterium tuberculosis | P9WPQ5 | - |
- |
6.3.3.3 | Mycobacterium tuberculosis H37Rv | P9WPQ5 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.3.3 | ATP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 | - |
Mycobacterium tuberculosis | ADP + phosphate + dethiobiotin + Na+ + OH- | - |
? | |
6.3.3.3 | ATP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 | - |
Escherichia coli | ADP + phosphate + dethiobiotin + Na+ + OH- | - |
? | |
6.3.3.3 | ATP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 | - |
Mycobacterium tuberculosis H37Rv | ADP + phosphate + dethiobiotin + Na+ + OH- | - |
? | |
6.3.3.3 | CTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 | - |
Mycobacterium tuberculosis | CDP + phosphate + dethiobiotin + Na+ + OH- | - |
? | |
6.3.3.3 | CTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 | - |
Escherichia coli | CDP + phosphate + dethiobiotin + Na+ + OH- | - |
? | |
6.3.3.3 | CTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 | - |
Mycobacterium tuberculosis H37Rv | CDP + phosphate + dethiobiotin + Na+ + OH- | - |
? | |
6.3.3.3 | GTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 | - |
Mycobacterium tuberculosis | GDP + phosphate + dethiobiotin + Na+ + OH- | - |
? | |
6.3.3.3 | GTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 | - |
Escherichia coli | GDP + phosphate + dethiobiotin + Na+ + OH- | - |
? | |
6.3.3.3 | GTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 | - |
Mycobacterium tuberculosis H37Rv | GDP + phosphate + dethiobiotin + Na+ + OH- | - |
? | |
6.3.3.3 | ITP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 | - |
Mycobacterium tuberculosis | IDP + phosphate + dethiobiotin + Na+ + OH- | - |
? | |
6.3.3.3 | ITP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 | - |
Escherichia coli | IDP + phosphate + dethiobiotin + Na+ + OH- | - |
? | |
6.3.3.3 | ITP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 | - |
Mycobacterium tuberculosis H37Rv | IDP + phosphate + dethiobiotin + Na+ + OH- | - |
? | |
6.3.3.3 | TTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 | - |
Mycobacterium tuberculosis | TDP + phosphate + dethiobiotin + Na+ + OH- | - |
? | |
6.3.3.3 | TTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 | - |
Escherichia coli | TDP + phosphate + dethiobiotin + Na+ + OH- | - |
? | |
6.3.3.3 | TTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 | - |
Mycobacterium tuberculosis H37Rv | TDP + phosphate + dethiobiotin + Na+ + OH- | - |
? | |
6.3.3.3 | UTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 | - |
Mycobacterium tuberculosis | UDP + phosphate + dethiobiotin + Na+ + OH- | - |
? | |
6.3.3.3 | UTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 | - |
Escherichia coli | UDP + phosphate + dethiobiotin + Na+ + OH- | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.3.3 | ? | x * 23500, SDS-PAGE | Mycobacterium tuberculosis |
6.3.3.3 | ? | x * 23500, SDS-PAGE | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.3.3 | BioD | - |
Mycobacterium tuberculosis |
6.3.3.3 | BioD1 | - |
Escherichia coli |
6.3.3.3 | DTBS | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.3.3 | 0.9 | - |
(7R,8S)-7,8-diaminopelargonic acid | mutant G169N, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 0.9 | - |
(7R,8S)-7,8-diaminopelargonic acid | mutant N175A, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 1 | - |
CTP | mutant G169N, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 1.2 | - |
GTP | mutant G169N, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 1.3 | - |
ATP | mutant G169N, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 1.7 | - |
NaHCO3 | mutant G169N, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 9.9 | - |
NaHCO3 | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 10.2 | - |
ITP | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 11.1 | - |
GTP | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 11.4 | - |
UTP | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 11.7 | - |
ATP | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 11.8 | - |
TTP | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 13.3 | - |
(7R,8S)-7,8-diaminopelargonic acid | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 15.2 | - |
CTP | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
6.3.3.3 | 15.4 | - |
NaHCO3 | mutant N175G, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 16.2 | - |
NaHCO3 | mutant N175A, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 16.3 | - |
GTP | mutant N175G, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 18.1 | - |
ATP | mutant N175A, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 18.3 | - |
GTP | mutant N175A, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 19.1 | - |
CTP | mutant N175A, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 19.6 | - |
CTP | mutant N175G, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 23.2 | - |
(7R,8S)-7,8-diaminopelargonic acid | mutant N175G, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 23.6 | - |
ATP | mutant N175G, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 28.1 | - |
CTP | wild-type, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 29 | - |
GTP | wild-type, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 38.4 | - |
NaHCO3 | wild-type, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 38.6 | - |
ATP | wild-type, pH 7.5, 37°C | Escherichia coli | |
6.3.3.3 | 49 | - |
(7R,8S)-7,8-diaminopelargonic acid | wild-type, pH 7.5, 37°C | Escherichia coli |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
6.3.3.3 | 0.054 | - |
wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | CTP | |
6.3.3.3 | 0.349 | - |
wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | ATP | |
6.3.3.3 | 0.437 | - |
wild-type, pH 7.5, 37°C | Escherichia coli | ATP | |
6.3.3.3 | 0.52 | - |
wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | ITP | |
6.3.3.3 | 0.597 | - |
wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | GTP | |
6.3.3.3 | 0.622 | - |
wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | TTP | |
6.3.3.3 | 0.634 | - |
wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | UTP | |
6.3.3.3 | 2 | - |
above 2.0, wild-type, pH 7.5, 37°C | Escherichia coli | GTP | |
6.3.3.3 | 5.752 | - |
wild-type, pH 7.5, 37°C | Escherichia coli | CTP |